Protective effect of C1 esterase inhibitor on reperfusion injury in the rat middle cerebral artery occlusion model
Protective effect of C1 esterase inhibitor on reperfusion injury in the rat middle cerebral artery occlusion model
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DOI：
10.1227/01.neu.0000043710.61233.b4
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发表时间：
2003-02-01
期刊：
影响因子：
4.8
通讯作者：
Sakaki, T
中科院分区：
文献类型：
--
作者：
Akita, N;Nakase, H;Sakaki, T
目的：补体系统被认为在启动再灌注损伤期间发生的一些炎症事件中起主要作用。本研究旨在探讨Cl酯酶抑制剂（C1-INH）对大鼠大脑中动脉缝合闭塞再灌注缺血损伤的影响。方法：选用雄性Wistar大鼠36只。大脑中动脉腔内闭塞60分钟。再灌注前给予C1-INH(50国际单位/kg) （C1-INH组，In = 19）或生理盐水溶液（对照组，n = 17）。在实验过程中记录生理参数（动脉血气值、平均动脉血压和心率）和局部脑血流量。再灌注48小时后，处死所有大鼠，用髓过氧化物酶活性测定法评估白细胞浸润情况，用2,3,5-三苯四氮氯化染色法进行组织学分析。结果：两组患者的生理参数及局部脑血流量值无显著差异。C1-INH组心肌梗死体积明显小于对照组（分别为84.9 +/- 69.1 mm(3)和0.40 +/- 0.29 units/g），髓过氧化物酶活性显著低于对照组（分别为202.3 +/- 98.3 mm(3)和1.41 +/- 0.44 units/g） （P < 0.01）。髓过氧化物酶活性与梗死体积呈正相关（r = 0.73, P < 0.01）。结论：C1-INH可减少局灶性缺血再灌注时多形核白细胞的积累和神经元的损伤。
OBJECTIVE: The complement system is thought to play a major role in initiating some of the inflammatory events that occur during reperfusion injury. The aim of this study was to assess the effects of Cl esterase inhibitor (C1-INH) on ischemic injury in the rat model of middle cerebral artery suture occlusion and reperfusion.METHODS: Thirty-six male Wistar rats were used. Intraluminal middle cerebral artery occlusion was performed for 60 minutes. just before reperfusion, C1-INH (50 international units/kg) (C1-INH group, In = 19) or saline solution (control group, n = 17) was administered. Physiological parameters (arterial blood gas values, mean arterial blood pressure, and heart rate) and local cerebral blood flow were recorded during the experiment. Forty-eight hours after reperfusion, all rats were killed, and assessments of leukocyte infiltration with a myeloperoxidase activity assay and histological analyses with 2,3,5-triphenyl tetrazolium chloride staining were performed.RESULTS: The physiological parameters and local cerebral blood flow values were not significantly different in the two groups. The infarction volume was significantly smaller and the myeloperoxidase activity was significantly lower in the C1-INH group (84.9 +/- 69.1 mm(3) and 0.40 +/- 0.29 units/g, respectively) than in the control group (202.3 +/- 98.3 mm(3) and 1.41 +/- 0.44 units/g, respectively) (P < 0.01). Myeloperoxidase activities were strongly correlated with infarction volumes (r = 0.73, P < 0.01).CONCLUSION: The results of this study indicated that C1-INH reduced polymorphonuclear leukocyte accumulation and neuronal damage in focal ischemia and reperfusion.