Agglutination of murine and guinea pig peritoneal cells by α‐L‐fucose‐binding lectin: Evonymus europaea
Agglutination of murine and guinea pig peritoneal cells by α‐L‐fucose‐binding lectin: Evonymus europaea
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α-L-岩藻糖结合凝集素对小鼠和豚鼠腹膜细胞的凝集：欧洲卫矛
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发表时间：
1983
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影响因子：
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通讯作者：
J. Podwińska
中科院分区：
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作者：
J. Petryniak;Danuta Duśa;J. Podwińska
在来自百脉根、欧洲荆豆I和欧洲的凝集素中，凝集细胞的血型H决定簇含有与亚末端D-半乳糖连接的L-岩藻糖α1→2-，只有最后一种凝集素凝集巯基乙酸盐和石蜡油刺激的小鼠和豚鼠腹膜渗出细胞（PEC）。凝集仅受Evelus凝集素的特异性抑制剂：乳糖-N-岩藻五糖I和乳糖抑制。这些结果表明PEC表面存在一个决定簇，在非还原端含有L-岩藻糖α1-连接，这与H血型决定簇不同。未受刺激的小鼠腹膜细胞（PC）不被凝集素凝集，但在神经氨酸酶处理后变得可凝集。来自不同动物的未受刺激的豚鼠PC被相同浓度的凝集素凝集到不同程度，并且在神经氨酸酶消化后显示出增加的凝集性。这些结果表明，在未受刺激的PC上也存在Evaluslectin受体，但唾液酸阻碍其进入。胰蛋白酶和链霉蛋白酶消化的PEC显示出与Evaculus凝集素的凝集性增加。这些结果表明，凝集素受体是糖脂。
Among lectins from Lotus tetragonolobus, Ulex europaeus I and Evonymus europaea, agglutinating cells with blood group H determinants containing L‐fucose α1→2‐linked to subterminal D‐galactose, only the last lectin agglutinates thioglycolate‐ and paraffin oil‐stimulated murine and guinea pig peritoneal exudate cells (PEC). The agglutination is inhibited by specific inhibitors of Evonymus lectin only: lacto‐N‐fucopentaose I and lactose. These results suggest the presence of a determinant on the surface of PEC, containing L‐fucose α1‐linked at the nonreducing end which is different from blood group H determinants. Nonstimulated murine peritoneal cells (PC) are not agglutinated by the lectin but become agglutinable after neuraminidase treatment. Unstimulated guinea pig PC from different animals are agglutinated to a different extent by the same lectin concentration and show increased agglutinability after neuraminidase digestion. These results show that receptor for Evonymus lectin also exists on the nonstimulated PC but access to it is hindered by sialic acid. Trypsin‐ and pronase‐digested PEC show increased agglutinability with Evonymus lectin. These results suggest that the lectin receptor is a glycolipid.