Eukaryotic picoplankton communities of the Mediterranean Sea in summer assessed by molecular approaches (DGGE, TTGE, QPCR)
Eukaryotic picoplankton communities of the Mediterranean Sea in summer assessed by molecular approaches (DGGE, TTGE, QPCR)
复制标题
DOI：
10.1111/j.1574-6941.2005.00058.x
复制
发表时间：
2006-03-01
影响因子：
4.2
通讯作者：
Vaulot, D
中科院分区：
文献类型：
--
作者：
Marie, D;Zhu, F;Vaulot, D
真核微型浮游生物（这里定义为小于3 μ m的细胞）的组成和丰度进行了研究，在摩洛哥的上升流和整个地中海在夏末使用流式细胞术和分子方法（梯度凝胶电泳和定量PCR）。微型浮游生物显示出典型的贫营养海洋区域的特征，在东部海盆的浓度低至1000个细胞mL（-1）。梯度凝胶电泳回收的最丰富的真核生物序列与未培养的海洋类群有关：alveolates I（16%）和II（26%）和一个新发现的类群（env南沙，17%），其序列最近已从中国南海获得，可能与珊瑚虫有关。Prasinophyceae（光合绿色藻类）占10%的序列，而尾藻，层藻，多囊藻，甲藻和纤毛虫提供了较小的贡献。使用定量PCR加上分类群特异性引物，使我们能够估计属于Prasinophyceae的几个分类群的相对丰度。在评估的三个属中，Bathycoccus似乎是最丰富的，在深度形成局部最大值。
The composition and abundance of eukaryotic picoplankton (defined here as cells smaller than 3 mu m) was investigated in the Morocco upwelling and throughout the Mediterranean Sea in late summer using flow cytometry and molecular methods (gradient gel electrophoresis and quantitative PCR). The picoplankton displayed characteristics typical of oligotrophic oceanic areas with concentrations down to 1000 cells mL(-1) in the Eastern Basin. The most abundant eukaryotic sequences recovered by gradient gel electrophoresis were related to uncultivated marine groups: alveolates I (16%) and II (26%) and a newly discovered group (env Nansha, 17%) for which sequences have been recently obtained from the South China Sea and that could be related to Acantharians. Prasinophyceae (photosynthetic green algae) accounted for 10% of the sequences, whereas Cercozoa, Stramenopiles, Polycystinea, dinoflagellates and ciliates provided minor contributions. The use of quantitative PCR coupled with taxon-specific primers allowed us to estimate the relative abundance of several taxa belonging to the Prasinophyceae. Of the three genera assessed, Bathycoccus appeared as the most abundant, forming localized maxima at depth.