Fur and iron transport proteins in the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius.
Fur and iron transport proteins in the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius.
复制标题
埃及流感嗜血杆菌生物群巴西紫癜克隆中的毛皮和铁转运蛋白。
DOI：
10.1099/00222615-48-7-629
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发表时间：
1999
影响因子：
3
通讯作者：
Actis,LA
中科院分区：
文献类型：
--
作者：
Smoot,LM;Bell,EC;Crosa,JH;Actis,LA
The Brazilian purpuric fever (BPF) clone ofHaemophilus influenzaebiogroup aegyptius causes a fatal septicaemic disease, resembling fulminant meningococcal sepsis, in children. When isolate F3031 was grown under iron-limiting conditions, the presence of several iron-regulated proteins of 38-110 kDa was revealed by electrophoretic analysis and a Fur homologue was shown by immunoblotting. Dot-blot assays and immunoblotting indicated that BPF cells bound human transferrin and contained transferrin-binding proteins in the outer membrane. However, the binding activity and the biosynthesis of these proteins were detected even under iron-rich conditions. Immunoblot analysis demonstrated the presence of a periplasmic protein related to the ferric iron-binding protein A (FbpA), the major iron-binding protein described inNeisseriaspp. However, the FbpA homologue in strain F3031 was constitutively expressed and was smaller than the periplasmic protein detected inH. influenzaetype b strain Eagan. The periplasm of strain F3031 also contained a protein related to theStreptococcus parasanguisFimA protein which recently has been shown to be involved in iron acquisition inYersinia pestis. Although the Eagan and F3031 FimA homologues had a similar mol. wt, of 31 kDa, the expression of the BPFfimA-like gene was not regulated by the iron concentration of the culture medium.
