Altered expression of insulin and insulin-like growth factor-I receptors in follicular and stromal compartments of polycystic ovaries.
Altered expression of insulin and insulin-like growth factor-I receptors in follicular and stromal compartments of polycystic ovaries.
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DOI：
10.1507/endocrj.40.413
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发表时间：
1993-08
影响因子：
2
通讯作者：
T. Samoto;T. Maruo;H. Matsuo;K. Katayama;E. Barnea;M. Mochizuki
中科院分区：
文献类型：
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作者：
T. Samoto;T. Maruo;H. Matsuo;K. Katayama;E. Barnea;M. Mochizuki
在卵巢中，胰岛素和胰岛素样生长因子-I（IGF-I）与FSH协同作用，增加颗粒细胞产生雌激素，与LH协同作用，增加卵泡膜基质细胞产生雄激素。胰岛素抵抗在多囊卵巢综合征（PCO）患者中也很常见。因此，在本研究中，我们研究了胰岛素和IGF-I受体在PCO卵巢的表达，并与正常卵巢进行了比较。卵巢组织取自4例行楔形切除术的PCO患者和6例行根治性子宫切除术的患者。免疫组织化学染色胰岛素和IGF-I受体进行抗生物素蛋白/生物素免疫过氧化物酶技术。在正常卵巢中，胰岛素和IGF-I受体在卵泡室中的表达在腔前卵泡阶段变得明显，并随着卵泡的生长而增强，而基质细胞，无论卵泡阶段，都具有胰岛素和IGF-I受体。在PCO卵巢与高胰岛素血症，没有胰岛素受体的表达，检测到颗粒或卵泡膜基质细胞，而IGF-I受体表达增加卵泡膜基质细胞，但减少颗粒细胞相比，在正常卵巢。然而，在PCO卵巢患者没有高胰岛素血症，胰岛素受体表达是明显的颗粒和卵泡膜基质细胞，与正常卵巢中观察到的强度相似，而IGF-I受体表达是可以忽略不计的颗粒细胞，但持续在卵泡膜基质细胞。这些结果表明，与高胰岛素血症相关的PCO卵巢胰岛素受体表达降低可能是继发于受体下调，而颗粒细胞表达缺陷沿着卵泡膜基质细胞IGF-I受体表达升高或持续表达可能在PCO卵巢中很常见，并有助于PCO的内分泌特征，其中不同程度的高雄激素血症是主要特征。
In the ovary, insulin and insulin-like growth factor-I (IGF-I) act synergistically with FSH to augment estrogen production by granulosa cells and with LH to augment androgen production by thecal stromal cells. It is also evident that insulin resistance is common in patients with polycystic ovary syndrome (PCO). Thus, in the present study we investigated the expression of insulin and IGF-I receptors in PCO ovaries and compared them with those in normal ovaries. Ovarian tissues were obtained from four PCO patients undergoing wedge resection, and from six patients who underwent radical hysterectomy. Immunohistochemical staining for insulin and IGF-I receptors was performed by avidin/biotin immunoperoxidase techniques. In normal ovaries, the expression of insulin and IGF-I receptors in follicular compartment became apparent in the preantral follicle stage and augmented with the follicular growth, while the stromal cells, regardless of the follicle stage, possessed insulin and IGF-I receptors. In PCO ovaries associated with hyperinsulinemia, no expression of insulin receptors was detected in granulosa or thecal stromal cells, while IGF-I receptor expression increased in thecal stromal cells but decreased in granulosa cells compared to those in normal ovaries. However, in PCO ovaries from patients without hyperinsulinemia, insulin receptor expression was apparent in both granulosa and thecal stromal cells, with a similar intensity to that observed in normal ovaries, while IGF-I receptor expression was negligible in granulosa cells but sustained in thecal stromal cells. These findings suggest that decreased expression of insulin receptors in PCO ovaries associated with hyperinsulinemia may be secondary to receptor down regulation, whereas defective expression in granulosa cells along with elevated or persisted expression in thecal stromal cells of IGF-I receptors may be common in PCO ovaries and contribute to the endocrine profiles of PCO in which varying degrees of hyperandrogenism is a predominant feature.