Characterization of a new 4BS.7HL wheat-barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat
Characterization of a new 4BS.7HL wheat-barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat
复制标题
DOI：
10.1139/g11-044
复制
发表时间：
2011-10-01
期刊：
影响因子：
3.1
通讯作者：
Molnar-Lang, M.
中科院分区：
文献类型：
--
作者：
Cseh, A.;Kruppa, K.;Molnar-Lang, M.
利用基因组原位杂交技术（GISH），在小麦新品种'Asakazekomugi' ×大麦'Manas'杂交后代中发现了自发的种间罗伯逊易位。荧光原位杂交（FISH）与重复的DNA序列（Afa家族，pSc119.2，和pTa 71）允许识别所有的小麦染色体，包括小麦染色体臂4 BS参与易位。利用大麦端粒和着丝粒特异性重复DNA探针（HvT 01和（AGGGAG）（n））进行的FISH证实了大麦染色体7 H的一个臂参与了易位。利用大麦7 H染色体长臂和短臂特异的SSR标记，将该易位片段鉴定为7 HL。对易位染色体的进一步分析阐明了7 H内遗传映射SSR的物理位置，特别关注其着丝粒区域。在7 HL的着丝粒区域中揭示了负责（1，3; 1，4）-β-D-葡聚糖产生的HvCslF 6基因的存在。增加（1，3;还在易位系中检测到（1，3; 1，4）-β-D-葡聚糖水平，表明HvCslF 6基因对于操纵小麦（1，3; 1，4）-β-D-葡聚糖水平具有潜在相关性。
A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat 'Asakaze komugi' x barley 'Manas' hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitive DNA probes (HvT01 and (AGGGAG)(n)) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. Further analysis of the translocation chromosome clarified the physical position of genetically mapped SSRs within 7H, with a special focus on its centromeric region. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-beta-D-glucan production, was revealed in the centromeric region of 7HL. An increased (1,3;1,4)-beta-D-glucan level was also detected in the translocation line, demonstrating that the HvCslF6 gene is of potential relevance for the manipulation of wheat (1,3;1,4)-beta-D-glucan levels.