Branched short elastin‐like peptides with temperature responsiveness obtained by EDTA‐mediated multimerization
Branched short elastin‐like peptides with temperature responsiveness obtained by EDTA‐mediated multimerization
复制标题
通过 EDTA 介导的多聚化获得具有温度响应性的支链短弹性蛋白样肽
DOI：
10.1002/psc.3449
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发表时间：
2022
影响因子：
2.1
通讯作者：
Nose Takeru
中科院分区：
文献类型：
--
作者：
Tanaka Naoki;Suyama Keitaro;Tomohara Keisuke;Maeda Iori;Nose Takeru
弹性蛋白样肽（ELPs）表现出可逆的相变，称为凝聚，由温度变化触发。这种特性使它们成为各种应用的刺激响应分子材料。在elp中，短肽链长度比长肽链长度有一些优势，因为短elp可以很容易地通过化学合成获得，允许在序列的任何位置使用各种氨基酸，包括D型和非天然氨基酸。此外，掺入的氨基酸很容易影响elp的温度响应行为。然而，为了在各种应用中得到利用，有必要开发短elp并研究它们的温度响应特性。为了进一步了解短ELP的温度响应行为，我们研究了由（FPGVG）n链（n= 1或2，分别缩写为F1和F2）组成的支链短ELP类似物。我们以乙二胺四乙酸（EDTA）为多聚体的中心组分，合成了由4条F1链或2至4条F2链组成的多聚体。我们的结果表明，得到的多聚体在水溶液中表现出凝聚性，而线性F1或F2则没有。此外，所得到的多定时器的结构特征与线性（FPGVG）4相同。在这项研究中，我们证明了能够凝聚的分子可以通过F1或F2的多聚化获得。利用短elp获得的温度响应分子使其成为易于合成的肽标签，从而赋予各种分子温度响应性，这将有助于开发具有多种功能的温度响应生物材料。
Elastin‐like peptides (ELPs) exhibit a reversible phase transition, known as coacervation, triggered by temperature changes. This property makes them useful as stimuli‐responsive molecular materials for various applications. Among ELPs, short peptide chain lengths have some advantages over long peptide chain lengths because short ELPs can be easily obtained by chemical synthesis, allowing the use of various amino acids, including D‐type and unnatural amino acids, at any position in the sequence. Moreover, the incorporated amino acids readily affect the temperature‐responsive behavior of ELPs. However, to be utilized in various applications, it is necessary to develop short ELPs and to investigate their temperature‐responsive properties. To obtain further insights into the temperature‐responsive behavior of the short ELPs, we investigated branched short ELP analogs composed of (FPGVG)nchains (n= 1 or 2, abbreviated as F1 and F2, respectively). We synthesized multimers composed of four F1 chains or two to four F2 chains using ethylenediaminetetraacetic acid (EDTA) as a central component of multimerization. Our results show that the multimers obtained exhibited coacervation in aqueous solutions whereas linear F1 or F2 did not. Furthermore, the structural features of the obtained multimers were the same as those of linear (FPGVG)4. In this study, we demonstrated that molecules capable of coacervation can be obtained by multimerization of F1 or F2. The temperature‐responsive molecules obtained using short ELPs make it possible to use them as easy‐to‐synthesize peptide tags to confer temperature responsiveness to various molecules, which will aid the development of temperature‐responsive biomaterials with a wide variety of functions.