Evidence of histamine receptors in fish brain using an in vivo [14C]2-deoxyglucose autoradiographic method and an in vitro receptor-binding autoradiographic method.
Evidence of histamine receptors in fish brain using an in vivo [14C]2-deoxyglucose autoradiographic method and an in vitro receptor-binding autoradiographic method.
复制标题
使用体内[14C]2-脱氧葡萄糖放射自显影方法和体外受体结合放射自显影方法证明鱼脑中的组胺受体。
DOI：
10.1016/s0013-9351(03)00111-7
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发表时间：
2004
影响因子：
8.3
通讯作者：
Silbergeld,EK
中科院分区：
文献类型：
--
作者：
Choich,JA;El-Nabawi,A;Silbergeld,EK
据推测，鱼具有功能性H1组胺受体，其具有结合H1组胺受体亚型特异性激动剂和拮抗剂的能力。对于这些实验，一种新的，在体内2-脱氧葡萄糖方法和标准的体外放射自显影程序的组合被利用。当鱼暴露于组胺能药物时，观察到神经功能的区域性、统计学显著剂量反应（即，H1激动剂和拮抗剂），这创造了第一个在鱼脑中H1组胺受体的神经学特征。选择H1组胺受体作为鱼类中的表征受体，因为组胺与多种神经功能相关，例如唤醒、注意力、感觉处理和认知的控制。组胺还在脑垂体激素分泌、食欲控制和潜在的前庭反应调节中起作用。此外，鱼脑在结构上有很好的特征，并且最近在斑马鱼中已经记录了H3样受体的存在。然而，到目前为止，很少有详细的信息，具体的本地化和功能的H1组胺受体在鱼类。
It was hypothesized that fish possess functioning H1 histamine receptors that have the ability to bind agonists and antagonists specific to the H1 histamine receptor subtype. For these experiments, a combination of a novel, in vivo 2-deoxyglucose method and a standard in vitro autoradiography procedure was utilized. A regional, statistically significant dose response in neurological functioning was observed when fish were exposed to histaminergic agents (i.e., H1 agonists and antagonists), which created the first neurological profile for the H1 histamine receptor in fish brain. The H1 histamine receptor was chosen as a characterization receptor in fish because histamine has been linked to a variety of neurological functions such as the control of arousal, attention, sensory processing, and cognition. Histamine also plays a role in pituitary hormone secretion, appetite control, and, potentially, regulation of vestigular reactivity. In addition, the fish brain is well characterized structurally, and the existence of an H3-like receptor has been documented recently in zebrafish. However, to date there is little detailed information about specific localization and functioning of the H1 histamine receptor in fish.