Bioactive Metabolites Produced by Penicillium sp.1 and sp.2, Two Endophytes Associated with Alibertia macrophylla (Rubiaceae)
Bioactive Metabolites Produced by Penicillium sp.1 and sp.2, Two Endophytes Associated with Alibertia macrophylla (Rubiaceae)
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DOI：
10.1515/znc-2009-11-1212
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发表时间：
2009-11
期刊：
影响因子：
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通讯作者：
C. M. Oliveira;G. Silva;L. Regasini;L. M. Zanardi;A. H. Evangelista;M. Young;V. Bolzani;Â. Araújo
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文献类型：
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作者：
C. M. Oliveira;G. Silva;L. Regasini;L. M. Zanardi;A. H. Evangelista;M. Young;V. Bolzani;Â. Araújo
在我们持续寻找来自巴西花卉植物中的内生真菌的生物活性代谢物的过程中，对大叶落葵（Rubiaceae）的叶进行内生真菌的分离，并分离出两种青霉菌。从青霉菌1的培养物的玉米中获得的乙腈级分提供苔黑酚（1）。另一方面，在马铃薯-葡萄糖-肉汤中培养的青霉菌1提供两种不同的化合物，环-（L-Pro-L-Val）（2）和尿嘧啶（3）。从青霉菌2获得的乙腈馏分的色谱分离导致三个二氢异香豆素，4-羟基melllein（4），8-甲氧基melllein（5）和5-羟基melllein（6）。化合物5和6为首次从青霉属中分离得到。此外，还评价了代谢产物1 - 6的抗真菌和乙酰胆碱酯酶（AChE）抑制活性。活性最强的化合物1和4对枝孢霉Cladosporiumcladosporioides和C. sphaerospermum。化合物2的检测限为10.0 μg，显示出有效的AChE抑制活性。
In the course of our continuous search for bioactive metabolites from endophytic fungi living in plants from the Brazilian fl ora, leaves of Alibertia macrophylla (Rubiaceae) were submitted to isolation of endophytes, and two species of Penicillium were isolated. The acetonitrile fraction obtained in corn from a culture of Penicillium sp.1 afforded orcinol (1). On the other hand, Penicillium sp.1 cultivated in potato-dextrose-broth furnished two different compounds, cyclo-(L-Pro-L-Val) (2) and uracil (3). The chromatographic fractionation of the acetonitrile fraction obtained from Penicillium sp.2 led to three dihydroisocoumarins, 4-hydroxymellein (4), 8-methoxymellein (5) and 5-hydroxymellein (6). Compounds 5 and 6 were obtained from the Penicillium genus for the fi rst time. Additionally, metabolites 1 - 6 were evaluated for their antifungal and acetylcholinesterase (AChE) inhibitory activities. The most active compounds 1 and 4 exhibited detection limits of 5.00 and 10.0 μg against Cladosporium cladosporioides and C. sphaerospermum, respectively. Compound 2 showed a detection limit of 10.0 μg, displaying potent AChE inhibitory activity.