CHANGES IN THE CYTOPLASMIC ELEMENTS OF CULTURED-CELLS INFECTED WITH EIMERIA-VERMIFORMIS SPOROZOITES
CHANGES IN THE CYTOPLASMIC ELEMENTS OF CULTURED-CELLS INFECTED WITH EIMERIA-VERMIFORMIS SPOROZOITES
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DOI：
10.1111/j.1550-7408.1989.tb01059.x
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发表时间：
1989-03-01
期刊：
影响因子：
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通讯作者：
BUSHELL, GR
中科院分区：
文献类型：
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作者：
ADAMS, JH;BUSHELL, GR
用纯化的蠕形艾美耳球虫子孢子接种上皮型(PK-15)和成纤维细胞型(MDBK)哺乳动物细胞。将接种后0~93h的配对样品进行电子显微镜观察，其中一半样品在固定前用非离子洗涤剂提取。对标本进行了透射和扫描电子显微镜检查。培养的细胞在2~93HAI之间有大量的孢子体附着，通常靠近细胞的边缘。一些寄主细胞微绒毛向上伸展并附着在子孢子上。在穿透过程中固定的子孢子在入侵处明显收缩，但在子孢子入侵或感染子孢子的细胞中，宿主细胞膜或表面微绒毛没有明显变化。在用1%Triton X-100提取的细胞中，宿主细胞骨架围绕寄生虫逐渐重组，但变化仅限于子孢子的直接区域。在驻留的子孢子周围，细胞骨架变得不那么致密，但也更有序，这与邻近的细胞区形成了对比。细胞骨架元素在寄生虫的上方和下方传递。细胞骨架的出现表明，寄主细胞在寄生虫周围形成了一个松散的篮状细胞骨架网络。
Epithelial-type (PK-15) and fibroblast-type (MDBK) mammalian cell cultures were inoculated with purified Eimeria vermiformis sporozoites. Matched samples from 0 to 93 h after inoculation (HAI) were processed for electron microscopy; half of the sample preparations were extracted with non-ionic detergent prior to fixation. Specimens were examined by both transmission and scanning electron microscopy. Numerous sprozoites were attached to the cultured cells from 2 to 93 HAI, usually near the cell periphery. Some host cell microvilli extended up and appeared attached to the sporozoites. Sporozoites fixed during the penetration process were markedly constricted at the site of entry; however, no noticeable changes occurred in the host cell membrane or surface microvilli during sporozoite invasion or in sporozoite-infected cells. In cells extracted with 1% Triton X-100, the host cytoskeleton was progressively reorganized about the parasites but changes were limited to the immediate area of the sporozoite. Around resident sporozoites, the cytoskeleton became less dense but also more ordered, which contrasted with adjacent cell areas. Cytoskeletal elements passed both over and under the parasites. The appearance of the cytoskeleton suggested that the host cell formed a loose, basket-like net of cytoskeletal elements about the parasite.