Isolation and characterization of two α chain size collagenous polypeptide chains c and d from glomerular basement membrane
Isolation and characterization of two α chain size collagenous polypeptide chains c and d from glomerular basement membrane
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肾小球基底膜中两条 α 链大小的胶原多肽链 c 和 d 的分离和表征
DOI：
10.1016/0014-5793(79)80536-0
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发表时间：
1979
期刊：
影响因子：
3.5
通讯作者：
S. Dixit
中科院分区：
文献类型：
--
作者：
S. Dixit
基底膜是由胶原蛋白(S)、一种或多种糖蛋白和~yco~-寡聚糖组成的细胞外结构[L-3]。目前，胶原蛋白的化学结构及其分子组成仍存在争议。基底膜的胶原成分(S)在遗传上构成了一类不同于I、II和III型胶原的胶原，其3-羟脯氨酸、羟赖氨酸、亮氨酸和碳水化合物的含量较高，而丙氨酸的含量较低。Kefaldes[4-61描述了一种链状大小的基底膜胶原组分(IV型)，从晶状体被膜、肾小球和Descemet膜的胃酶消化中获得。Timpl等人[7]估计IV型胶原的相对分子质量为40万。其他研究人员发表的研究结果表明，肾小球基底膜的胶原性成分具有异质性。Q链大小的多肽链A和B已经从胎盘、肝脏、皮肤和血管组织的中层中分离出来[1 11 31.类似的链A和B也在胎盘[14]，牛骨骼肌[151，滑膜和*命名法]中被报道：本文提出的C和D链的命名法对应于Kresina，Rhodes和Miller(1978)Fed使用的命名法。程序FASE B 37(6)，1528.Paul Bornstein博士还将C和D链分别称为CuL(IV)和&(IV)链
Basement membranes are extracellular structures composed of collagenous protein (s), one or more glycoproteins and~ yco~ minoglycans [l-3]. At the present time, the chemical structure of the collagenous component and its molecular organization remain controversial. The collagen component (s) of basement membrane constitutes a genetically distinct class of collagen differing from types I, II and III collagen by the presence of higher contents of 3-hydroxyproline, hydroxylysine, leucine, and carbohydrate and low contents of alanine. Kefalides [4-61 described an (II chain size basement membrane collagenous component (type IV), obtained from the pepsin digest of lens capsule, glomerulus, and descemet’s membrane. Timpl et al.[7] have estimated that molecular weight of type IV collagen is> 400 000. Other investigators [&lo] have published results indicating the heterogenous nature of the collagenous component of glomerular basement membrane. Q Chain size polypeptide chains A and B have been isolated from placenta, liver, skin, and the medial layer of vascular tissues [1 ll 31. Similar chains A and B have also been reported from placenta [14], bovine skeletal muscle [151, synovial membrane and* Nomenclature: The nomenclature of C and D chains presented in this paper corresponds to one used by Kresina, Rhodes and Miller (1978) Fed. Proc. FASEB 37 (6), 1528. The C and D chains have also been referred to as cul (IV) and & (IV) chains, respectively, by Dr Paul Bornstein