Preparation of extracellular matrices produced by cultured and primary fibroblasts.
Preparation of extracellular matrices produced by cultured and primary fibroblasts.
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DOI：
10.1002/0471143030.cb1009s33
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Cukierman, Edna
中科院分区：
文献类型：
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作者：
Beacham, Dorothy A;Amatangelo, Michael D;Cukierman, Edna
成纤维细胞分泌和组织细胞外基质（ECM），其除了调节细胞功能如生长和存活之外，还为其粘附、迁移和组织组织化提供结构支持。细胞与基质的相互作用对脊椎动物的发育至关重要。这些过程中的障碍与纤维化、发育畸形、癌症和其他疾病有关。本单元描述了一种制备NIH-3 T3细胞衍生的三维基质的方法;该基质是三维的，无细胞和碎片，并附着在二维培养表面上。细胞粘附和扩散在这些基质上是正常的。该基质也可以压缩成二维基质并溶解以生物化学地研究基质。
Fibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interactions are vital for vertebrate development. Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit describes a method for preparing a three-dimensional matrix derived from NIH-3T3 cells; the matrix is three-dimensions, cell and debris free, and attached to a two-dimensional culture surface. Cell adhesion and spreading are normal on these matrices. This matrix can also be compressed into a two-dimensional matrix and solubilized to study the matrix biochemically.