Topogenesis of membrane proteins: determinants and dynamics
Topogenesis of membrane proteins: determinants and dynamics
复制标题
DOI：
10.1016/s0014-5793(01)02712-0
复制
发表时间：
2001-08-31
期刊：
影响因子：
3.5
通讯作者：
Spiess, M
中科院分区：
文献类型：
--
作者：
Goder, V;Spiess, M
为了将蛋白质靶向和整合到哺乳动物内质网中，可以区分两种类型的信号：易位其C-末端序列的信号（可切割信号和信号锚）和易位其N-末端的信号（反向信号锚）。除了侧翼电荷的良好建立的作用之外，信号的非极性核心的长度和疏水性以及蛋白质折叠和糖基化也有助于在易位子中定向信号。在多跨膜蛋白中，拓扑决定簇分布在整个序列中，甚至可能相互竞争。在拓扑发生过程中，多达60个残基的片段可以通过易位子来回移动，强调了拓扑发生的意想不到的动态方面。(C)2001年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
For targeting and integration of proteins into the mammalian endoplasmic reticulum, two types of signals can be distinguished: those that translocate their C-terminal sequence (cleavable signals and signal-anchors) and those that translocate their N-terminus (reverse signal-anchors). In addition to the well established effect of flanking charges, also the length and hydrophobicity of the apolar core of the signal as well as protein folding and glycosylation contribute to orienting the signal in the translocon. In multi-spanning membrane proteins, topogenic determinants are distributed throughout the sequence and may even compete with each other. During topogenesis, segments of up to 60 residues may move back and forth through the translocon, emphasizing unexpected dynamic aspects of topogenesis. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.