Genomic subtraction followed by dot blot screening of Streptococcus pneumoniae clinical and carriage isolates identifies genetic differences associated with strains that cause otitis media.
Genomic subtraction followed by dot blot screening of Streptococcus pneumoniae clinical and carriage isolates identifies genetic differences associated with strains that cause otitis media.
复制标题
对肺炎链球菌临床和携带菌株进行基因组消减，然后进行斑点印迹筛选，以确定与引起中耳炎的菌株相关的遗传差异。
DOI：
10.1128/iai.73.5.2805-2811.2005
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发表时间：
2005
影响因子：
3.1
通讯作者：
Fennie,KristopherP
中科院分区：
文献类型：
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作者：
Pettigrew,MelindaM;Fennie,KristopherP
肺炎链球菌是细菌性中耳炎的主要原因，但对这种疾病的特定细菌因素知之甚少。我们使用了分子流行病学方法，包括基因组减去这些。肺炎血清19组中耳菌株5093与实验室菌株R6比较。用所得的减法PCR （sPCR）产品筛选来自幼儿的93个中耳分离株、90个血液分离株、35个载体分离株和58个脑脊液分离株，以鉴定中耳分离株中常见的基因。探针P41类似于一种假设的梅利氏布鲁氏菌蛋白，在41%的中耳分离株中出现，在中耳菌株中出现的频率分别是携带株、血液株和脑膜炎株的2.8倍（95%可信区间[CI]， 1.32 ~ 6.5）、3.3倍（95% CI, 1.9 ~ 5.7）和1.8倍（95% CI, 1.1 ~ 3.0）。sPCR片段H10，类似于未知的无乳链球菌蛋白，在31%的中耳分离株中存在，在中耳分离株中分别出现3.6 （95% CI， 1.2至11.2）、2.8 （95% CI， 1.5至5.4）和2.6 （95% CI， 1.2至5.5）倍于携带株、血株或脑膜炎株。这些研究已经确定了两个在中耳炎毒性中具有潜在重要性的基因。需要进一步的研究来确定这些基因在中耳炎发病机制中的确切作用。
Streptococcus pneumoniaestrains are the leading cause of bacterial otitis media, yet little is known about specific bacterial factors important for this disease. We utilized a molecular epidemiological approach involving genomic subtraction of theS. pneumoniaeserogroup 19 middle ear strain 5093 against the laboratory strain R6. Resulting subtraction PCR (sPCR) products were used to screen a panel of 93 middle ear, 90 blood, 35 carriage, and 58 cerebrospinal fluid isolates from young children to identify genes found more frequently among middle ear isolates. Probe P41, similar to a hypothetical protein ofBrucella melitensis, occurred among 41% of middle ear isolates and was found 2.8 (95% confidence interval [CI], 1.32 to 6.5), 3.3 (95% CI, 1.9 to 5.7), and 1.8 (95% CI, 1.1 to 3.0) times more frequently among middle ear strains than carriage, blood, or meningitis strains, respectively. sPCR fragment H10, similar to an unknownStreptococcus agalactiaeprotein, was present in 31% of middle ear isolates and occurred 3.6 (95% CI, 1.2 to 11.2), 2.8 (95% CI, 1.5 to 5.4), and 2.6 (95% CI, 1.2 to 5.5) times more often among middle ear isolates than carriage, blood, or meningitis strains, respectively. These studies have identified two genes of potential importance in otitis media virulence. Further studies are warranted to outline the precise role of these genes in otitis media pathogenesis.