MITE-transposon display efficiently detects polymorphisms among the Oryza AA-genome species
MITE-transposon display efficiently detects polymorphisms among the Oryza AA-genome species
复制标题
DOI：
10.1270/jsbbs.53.125
复制
发表时间：
2003-06-01
期刊：
影响因子：
2.4
通讯作者：
Sano, Y
中科院分区：
文献类型：
--
作者：
Takagi, K;Nagano, H;Sano, Y
微型反向重复转座元件（MITEs）是水稻基因组中散布的重复序列的主要组成部分。这些元件是在整个基因组中进行精细基因组分析的极好工具。利用水稻基因组数据库，我们评估了MITE存在于一个200 kb的区域周围的水稻糯性位点，并选择了四个MITE的转座子展示（MITE TD）分析亚科。MITE-TD是一种基于MITE序列的AFLP相关技术，利用所选的4个MITEs检测AA基因组稻属物种之间的多态性。在这里使用的MITE-TD能够最有效地检测到的所有分子标记技术的多态性应用到稻属物种。Mashu是一个新的MITE家族，其检测频率是其他三种MITE的3-1.5倍，是检测多态性的最佳系统。MITE-TDs还揭示了AA基因组物种内遗传变异的信息，以及O. sativa和O.普通猪毛菜
Miniature inverted repeat transposable elements (MITEs) are a major component of interspersed repetitive sequences in the rice genome. These elements serve as excellent tools for fine genomic analysis throughout the genome. Using the rice genome database, we evaluated MITEs present in a 200-kb region surrounding the rice waxy locus and selected four MITE subfamilies for MITE-transposon display (MITE-TD) analyses. MITE-TD, which is an AFLP-related technique based on MITE sequences, was applied to detect polymorphisms among the AA-genome Oryza species using the four selected MITEs. The MITE-TD used here enabled the most efficient detection of polymorphisms of all the molecular marker techniques applied to date in the Oryza species. Of the four MITEs, Mashu, a new MITE family, was found to be the best system for detecting the polymorphisms, with a detection frequency 3-1.5 times higher than that of the other three MITEs. The MITE-TDs also revealed information about genetic variations within the AA-genome species, and the complexity of the genetic relationships between O. sativa and O. rufipogon.