Apigenin-7-diglucuronide protects retinas against bright light-induced photoreceptor degeneration through the inhibition of retinal oxidative stress and inflammation
Apigenin-7-diglucuronide protects retinas against bright light-induced photoreceptor degeneration through the inhibition of retinal oxidative stress and inflammation
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Apigenin-7-diglucuronide 通过抑制视网膜氧化应激和炎症来保护视网膜免受强光诱导的光感受器变性
DOI：
10.1016/j.brainres.2017.03.019
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发表时间：
2017-05-15
期刊：
影响因子：
2.9
通讯作者：
Chen, Yu
中科院分区：
文献类型：
--
作者：
Bian, Minjuan;Zhang, Yong;Chen, Yu
视网膜退行性疾病如年龄相关性黄斑变性中的视力损害主要与光感受器变性相关，其中氧化应激和炎症反应作为中心参与者在机制上参与。具有光感受器保护特性的疗法仍有待开发。芹菜素-7-二葡糖苷酸（A7 DG）是一种黄酮苷类化合物，存在于多种药用植物中，具有抗炎或抗氧化活性。然而，A7 DG的药理学意义在体内仍然未知。本研究从Glecelumba（Nakai）Kuprian中分离A7 DG，并研究A7 DG对以强光诱导的光感受器变性为特征的小鼠的视网膜保护作用。结果表明，A7 DG处理导致了明显的光暴露的BALB/c小鼠的感光细胞保护。此外，A7 DG治疗减轻感光细胞凋亡，减轻氧化应激，抑制反应性神经胶质增生和小胶质细胞活化，并减弱在明亮的光暴露的视网膜中的促炎基因的表达。实验结果首次证实了A7 DG在体内具有明显的光感受器保护活性。A7 DG对强光诱导的视网膜氧化应激和视网膜炎症反应的抑制与其对视网膜的保护作用有关。这项工作保证了A7 DG作为治疗威胁视力的视网膜退行性疾病的候选药物的进一步评价。此外，鉴于氧化应激和炎症在神经退行性疾病发病机制中的普遍意义，A7 DG可以进一步测试用于治疗其他神经退行性疾病。(C)2017爱思唯尔B. V.保留所有权利。
Vision impairment in retinal degenerative diseases such as age-related macular degeneration is primarily associated with photoreceptor degeneration, in which oxidative stress and inflammatory responses are mechanistically involved as central players. Therapies with photoreceptor protective properties remain to be developed. Apigenin-7-diglucuronide (A7DG), a flavonoid glycoside, is present in an assortment of medicinal plants with anti-inflammatory or ant-oxidant activities. However, the pharmacological significance of A7DG remains unknown in vivo. The current study isolated A7DG from Glechoma longituba (Nakai) Kuprian and investigated the retinal protective effect A7DG in mice characterized by bright light-induced photoreceptor degeneration. The results showed that A7DG treatment led to remarkable photoreceptor protection in bright light-exposed BALB/c mice. Moreover, A7DG treatment alleviated photoreceptor apoptosis, mitigated oxidative stress, suppressed reactive gliosis and microglial activation and attenuated the expression of proinflammatory genes in bright light-exposed retinas. The results demonstrated for the first time remarkable photoreceptor protective activities of A7DG in vivo. Inhibition of bright light-induced retinal oxidative stress and retinal inflammatory responses was associated with the retinal protection conferred by A7DG. The work here warrants further evaluation of A7DG as a pharmacological candidate for the treatment of vision-threatening retinal degenerative disorders. Moreover, given the general implication of oxidative stress and inflammation in the pathogenesis of neurodegeneration, A7DG could be further tested for the treatment of other neurodegenerative disorders. (C) 2017 Elsevier B.V. All rights reserved.