Skeletal muscle of female rats exhibit higher mitochondrial mass and oxidative-phosphorylative capacities compared to males
Skeletal muscle of female rats exhibit higher mitochondrial mass and oxidative-phosphorylative capacities compared to males
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DOI：
10.1159/000099208
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Garcia-Palmer, Francisco J.
中科院分区：
文献类型：
--
作者：
Colom, Bartomeu;Alcolea, Maria Pilar;Garcia-Palmer, Francisco J.
性别和热量限制对大鼠腓肠肌线粒体含量和氧化磷酸化能力的影响进行了研究。肌肉蛋白质、线粒体蛋白质和DNA含量、线粒体氧化和磷酸化系统的酶活性、线粒体抗氧化酶、复合物IV的蛋白质水平（亚基I和IV）和ATP酶，以及参与线粒体复制和转录的线粒体转录因子A（TFAM）的基因和蛋白表达，在自由进食并进行三个月的40%热量限制的两种性别的大鼠中测量。与雄性大鼠相比，雌性大鼠腓肠肌的线粒体DNA和蛋白质含量，TFAM蛋白水平，氧化和磷酸化机制和活动，谷胱甘肽过氧化物酶活性较高。总之，目前的数据显示了一个明确的性别二型性大鼠肌肉线粒体功能，这可以解释更高的设施，以适应改变代谢能量的情况。版权所有（c）2007 S. Karger AG，巴塞尔。
The effect of gender and caloric restriction on mitochondrial content and oxidative-phosphorylative capacities has been investigated in rat gastrocnemius muscle. Muscle protein, mitochondrial protein and DNA contents, enzymatic activities of mitochondrial oxidative and phosphorylative system, mitochondrial antioxidant enzymes, protein levels of complex IV (subunit I and IV) and ATPase, and the gene and protein expression of mitochondrial transcription factor A (TFAM), involved in mitochondrial replication and transcription, were measured in rats of both genders fed ad libitum and subjected to three months of 40% caloric restriction. Compared to males, gastrocnemius muscle of female rats showed higher mitochondrial DNA and protein contents, TFAM protein level, oxidative and phosphorylative machinery and activities, and glutathione peroxidase activity. In conclusion, the present data show a clear gender dimorphism in rat muscle mitochondrial features, which could explain the higher facility of females to adapt to altered metabolic energy situations. Copyright (c) 2007 S. Karger AG, Basel.