AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants
AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants
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DOI：
10.1128/jb.178.1.306-308.1996
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发表时间：
1996-01-01
影响因子：
3.2
通讯作者：
Nikaido, H
中科院分区：
文献类型：
--
作者：
Okusu, H;Ma, D;Nikaido, H
大肠杆菌的多重抗生素抗性（Mar）突变体对多种抗生素具有抗性，并且已知增加的主动外排是导致对某些药物的抗性的原因。然而，外排系统的身份仍然未知。通过构建一个等基因组。大肠杆菌K-12菌株？我们表明，在缺乏AcrAB外排系统的情况下，marR 1突变不能增加抗性水平。该实验鉴定了AcrAB系统是负责使Mar突变体对许多药剂（包括四环素、氯霉素、氨苄青霉素、萘啶酸和利福平）产生抗性的主要泵。
Multiple-antibiotic-resistance (Mar) mutants of Escherichia coli are resistant to a wide variety of antibiotics, and increased active efflux is known to be responsible for the resistance to some drugs. The identity of the efflux system, however, has remained unknown. By constructing an isogenic set off. coli K-12 strains? we showed that the marR1 mutation was incapable of increasing the resistance level in the absence of the AcrAB efflux system, This experiment identified the AcrAB system as the major pump responsible for making the Mar mutants resistant to many agents, including tetracycline, chloramphenicol, ampicillin, nalidixic acid, and rifampin.