Comprehensive and innovative techniques for liver transplantation in rats: A surgical guide
Comprehensive and innovative techniques for liver transplantation in rats: A surgical guide
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DOI：
10.3748/wjg.v16.i25.3120
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发表时间：
2010-07-07
影响因子：
4.3
通讯作者：
Uemoto, Shinji
中科院分区：
文献类型：
--
作者：
Hori, Tomohide;Nguyen, Justin H.;Uemoto, Shinji
目的：探讨大鼠原位肝移植（orthopoticlivertransplantation，奥尔特）的学习曲线及手术成功的最重要因素。方法：描述大鼠奥尔特模型的手术过程，测定操作者的学习曲线。确定了促成手术成功的各种因素。最重要的手术因素之间进行了评估成功和不成功的surgics.RESULTS：学习曲线数据表明，50例需要操作员培训开始研究。单因素分析显示，手术时间、失血量、热缺血时间、无肝期、不稳定的全身血流动力学状态、术后体温对手术成功率有显著影响，而多因素分析显示，无肝期是影响手术成功率的最关键因素。缩短无肝期是该模型成功的关键。(C)2010年百世登。All rights reserved.
AIM: To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery.METHODS: We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.RESULTS: Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynannic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis.CONCLUSION: OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model. (C) 2010 Baishideng. All rights reserved.