A new family of adenylyl cyclase genes in the male germline of Drosophila melanogaster
A new family of adenylyl cyclase genes in the male germline of Drosophila melanogaster
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DOI：
10.1007/s004270050304
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发表时间：
2000-04-01
影响因子：
2.4
通讯作者：
Levin, LR
中科院分区：
文献类型：
--
作者：
Cann, MJ;Chung, E;Levin, LR
我们描述了一个新的果蝇腺苷酸环化酶相关基因家族的克隆和特征。定义这个家族的5个腺苷酸环化酶样基因与先前已知的腺苷酸环化酶明显不同。一个成员在第3号染色体上形成一个独特的位点，而其他四个成员在第2号染色体上形成一个紧密聚集的串联重复阵列。2号染色体上的基因在雄性生殖系中以双性依赖的方式转录，并在有丝分裂后、减数分裂和早期分化的精子中表达。因此，这些基因为cAMP信号通路在果蝇精子发生中的作用提供了第一个证据。该基因座的表达受精子发生过程中减数分裂I的G(2)/M转变所必需的总是早基因、炮弹基因、减数分裂停止基因和精母细胞停止基因的控制，这意味着分化和增殖的协调机制。在2号染色体上的正选择也提供了证据，这表明该基因家族正在积极进化，并可能在精子发生中发挥新的作用。
We describe the cloning and characterization of a new gene family of adenylyl cyclase related genes in Drosophila. The five adenylyl cyclase-like genes that define this family are clearly distinct from previously known adenylyl cyclases. One member forms a unique locus on chromosome 3 whereas the other four members form a tightly clustered, tandemly repeated array on chromosome 2. The genes on chromosome 2 are transcribed in the male germline in a doublesex, dependent manner and are expressed in postmitotic, meiotic, and early differentiating sperm. These genes therefore provide the first evidence for a role for the cAMP signaling pathway in Drosophila spermatogenesis. Expression from this locus is under the control of the always early, cannonball, meiosis arrest, and spermatocyte arrest genes that are required for the G(2)/M transition of meiosis I during spermatogenesis, implying a mechanism for the coordination of differentiation and proliferation. Evidence is also provided for positive selection at the locus on chromosome 2 which suggests this gene family is actively evolving and may play a novel role in spermatogenesis.