Reactive Oxygen Species Scavenging Activity of Jixueteng Evaluated by Electron Spin Resonance (ESR) and Photon Emission
Reactive Oxygen Species Scavenging Activity of Jixueteng Evaluated by Electron Spin Resonance (ESR) and Photon Emission
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DOI：
10.1177/1934578x1400901225
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发表时间：
2014-12
影响因子：
1.8
通讯作者：
T. Toyama;S. Wada-Takahashi;Maomi Takamichi;Kiyoko Watanabe;Ayaka Yoshida;Fumihiko Yoshino;C. Miyamoto;Y. Maehata;Shuta Sugiyama;Shun-suke Takahashi;K. Todoki;Masaichi-chang-il Lee;N. Hamada
中科院分区：
文献类型：
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作者：
T. Toyama;S. Wada-Takahashi;Maomi Takamichi;Kiyoko Watanabe;Ayaka Yoshida;Fumihiko Yoshino;C. Miyamoto;Y. Maehata;Shuta Sugiyama;Shun-suke Takahashi;K. Todoki;Masaichi-chang-il Lee;N. Hamada
鸡血藤是豆科植物鸡血藤的干燥茎，是一种传统的中草药，通常被归类为活血药，可用于治疗血瘀症。本研究的目的是考察积血藤等中草药对活性氧的清除作用。采用电子自旋共振(ESR)技术和微弱发光方法研究了鸡血藤、蛇床子水提物和丹参水提物对ROS的清除活性。鸡血藤对超氧阴离子(O2·-)和羟基自由基(HO·)的ESR信号强度的抑制作用较其他中草药更强。利用XYZ化学发光系统作为微弱发光测量和分析系统，观察到鸡血藤对过氧化氢(H_2O_2)和HO·的高发光强度。本研究结果表明，8%积血藤对ROS的清除活性最强。据报道，鸡血藤中含有多种多酚类物质。在鸡血藤清除ROS的活性中，推测这些多酚类物质的抗氧化活性可能有很大的贡献。综上所述，鸡血藤水提物组分具有较强的清除自由基活性和抑制O2·-、H2O2、HO·氧化作用的抗氧化作用。因此，肌血腾是一种很有前途的治疗活性氧相关疾病的药物。
Jixueteng, the dried stem of Spatholobus suberectus Dunn (Leguminosae), is a traditional Chinese herbal medicine that is commonly classified as a herb that promotes blood circulation and can be used to treat blood stasis. The aim of this study was to examine the reactive oxygen species (ROS) scavenging activity of Jixueteng and other herbal medicines. The ROS scavenging activities of the water extracts of Jixueteng, Cnidium officinale and Salvia miltiorrhiza were examined using an electron spin resonance (ESR) technique and faint luminescence measurement. The ESR signal intensities of the superoxide anion (O2•-) and hydroxyl radical (HO•) were reduced more by Jixueteng than the other herbal medicines we tested. High photon emission intensity to hydrogen peroxide (H2O2) and HO• was observed in Jixueteng using the XYZ chemiluminescence system that was used as faint luminescence measurement and analysis. The results of the present study revealed that the ROS scavenging activity of 8% Jixueteng was the strongest among the herbal medicines we tested. It has been reported that Jixueteng includes various polyphenols. In the ROS scavenging activity by Jixueteng, it is supposed that the antioxidant activity caused by these polyphenols would contribute greatly. In conclusion, a water extract component of Jixueteng had potent free radical scavenging activity and an antioxidative effect that inhibited the oxidative actions of O2•-, H2O2 and HO•. Therefore, Jixueteng represents a promising therapeutic drug for reactive oxygen-associated pathologies.