COMPARATIVE HISTOLOGICAL, HISTOCHEMICAL, IMMUNOHISTOCHEMICAL AND BIOCHEMICAL-STUDIES ON ESTROGEN-RECEPTORS, LECTIN RECEPTORS, AND BARR BODIES IN HUMAN-BREAST CANCER
COMPARATIVE HISTOLOGICAL, HISTOCHEMICAL, IMMUNOHISTOCHEMICAL AND BIOCHEMICAL-STUDIES ON ESTROGEN-RECEPTORS, LECTIN RECEPTORS, AND BARR BODIES IN HUMAN-BREAST CANCER
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DOI：
10.1007/bf00708323
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
SCHEIDT, E
中科院分区：
文献类型：
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作者：
REMMELE, W;HILDEBRAND, U;SCHEIDT, E
本研究共对567例女性乳腺癌患者进行了生化检测，（葡聚糖-包被活性炭法= DCC）检测雌激素受体（ER）的方法与其它一些用于检测ER或预测预后的形态学方法进行了比较（FSA =荧光配体结合测定，ER-ICA = ER的单克隆抗体测定，LRA =使用花生凝集素的凝集素受体测定，和巴尔体估计）。而在所有的DCC和FSA和巴尔体估计的结果之间没有观察到相关性，ER-ICA和LRA表现出一致的趋势，随着染色产物的强度增加，ER值更高。ER-ICA的结果可以通过由染色强度（SI）和阳性细胞百分比（PP）计算的免疫反应评分（IRS）表示。形态学的方法进行评估，特别考虑到他们的相关性与DCC，他们的理论基础，和他们的实际应用。总之，ER-ICA似乎是直接可视化ER蛋白的唯一方法，与DCC相反，因此完全不依赖于肿瘤组织中内源性或外源性雌激素的含量。LRA提供了关于肿瘤分化和对内分泌治疗的可能反应的有价值的额外信息，而FSA和巴尔体估计应被认为是过时的，因此应被放弃。
The present study performed on a total of 567 cases of human female breast cancer compares the results of the biochemical assay (dextran-coated charcoal assay = DCC) for oestrogen receptor (ER) with those of several morphological methods developed for the detection of the ER or for the predication of prognosis by use of other systems (FSA = fluorescent ligand binding assay, ER-ICA = monoclonal antibody assay for ER, LRA = lectin receptor assay using peanut agglutinin, and Barr body estimation). Whereas no correlation at all was observed among the results of the DCC and those of the FSA and Barr body estimation, the ER-ICA and the LRA showed an unanimous tendency towards higher values of ER with increasing intensity of the staining product. The results of the ER-ICA may be expressed by an immuno-reactive score (IRS) calculated from the staining intensity (SI) and the percentage of positive cells (PP). The morphological methods are evaluated with special regard to their correlation with the DCC, their theoretical basis, and their practical application. In summary, the ER-ICA appears to be the sole method directly visualizing the ER protein and - in contrast to the DCC - is therefore completely independent of the content of endogenous or exogenous oestrogens in the tumor tissue. The LRA provides valuable additional information concerning tumour differentiation and possible response to endocrine therapy, whereas the FSA and Barr body estimation should be considered as obsolete and should therefore be abandoned.