Inhibitory effects of anti‐rheumatic drugs on vascular endothelial growth factor in cultured rheumatoid synovial cells
Inhibitory effects of anti‐rheumatic drugs on vascular endothelial growth factor in cultured rheumatoid synovial cells
复制标题
抗风湿药物对培养类风湿滑膜细胞血管内皮生长因子的抑制作用
DOI：
--
复制
发表时间：
1999
影响因子：
4.6
通讯作者：
Minoru Sasano
中科院分区：
文献类型：
--
作者：
M. Nagashima;S. Yoshino;Hiroyuki Aono;M. Takai;Minoru Sasano
血管内皮生长因子（VEGF）是一种强有力的血管生成诱导剂，在类风湿关节炎（RA）的滑膜中组成性表达。VEGF的过度表达可能在RA的病理血管化和滑膜增生中起重要作用。在本研究中，我们检查了疾病缓解抗风湿药物（DMARD），包括布西拉明（布克），硫代苹果酸金钠（GST），甲氨蝶呤（MTX）和柳氮磺胺吡啶（SASP），是否通过抑制RA患者培养的滑膜细胞产生VEGF来发挥作用。用脂多糖（LPS）处理培养的滑膜细胞显著增加了培养的滑膜细胞的VEGF产生。布克显著抑制LPS诱导的VEGF产生，而GST倾向于抑制VEGF的产生。对VEGF产生的抑制作用具有剂量依赖性。相反，MTX和SASP不影响VEGF的产生。逆转录-聚合酶链反应（RT-PCR）分析显示，布克也抑制LPS诱导的RA滑膜细胞VEGF mRNA表达。本研究首次证明布克抑制类风湿关节炎患者滑膜细胞VEGF的产生及其mRNA的表达。我们的研究结果表明，布克的抗风湿作用是通过抑制滑膜细胞产生VEGF来抑制RA滑膜中的血管生成和滑膜增殖。
Vascular endothelial growth factor (VEGF) is a potent inducer of angiogenesis and is constitutively expressed in the synovium of rheumatoid arthritis (RA). Over‐expression of VEGF may play an important role in pathogenic vascularization and synovial hyperplasia of RA. In the present study, we examined whether disease‐modifying anti‐rheumatic drugs (DMARDs), including bucillamine (BUC), gold sodium thiomalate (GST), methotrexate (MTX) and salazosulfapiridine (SASP), act by inhibiting the production of VEGF by cultured synovial cells of patients with RA. Treatment of cultured synoviocytes with lipopolysaccharide (LPS) significantly increased VEGF production by cultured synovial cells. BUC significantly inhibited LPS‐induced VEGF production, while GST tended to inhibit the production of VEGF. The inhibitory effects on VEGF production were dose‐dependent. In contrast, MTX and SASP did not affect VEGF production. Reverse transcriptase‐polymerase chain reaction (RT‐PCR) analysis showed that BUC also inhibited LPS‐induced VEGF mRNA expression in RA synovial cells. The present study provides the first evidence that BUC inhibits VEGF production and the expression of its mRNA in synovial cells of RA patients. Our results indicate that the anti‐rheumatic effects of BUC are mediated by suppression of angiogenesis and synovial proliferation in the RA synovium through the inhibition of VEGF production by synovial cells.
疾病缓解抗风湿药物布西拉明的免疫抑制：布西拉明及其代谢物抑制人 T 淋巴细胞功能。
DOI：
--
发表时间：
1993
期刊：
The Journal of rheumatology
影响因子：
--
作者：
Hashimoto,K;Lipsky,PE
通讯作者：
Lipsky,PE
DOI：
10.1186/s12940-016-0123-2
发表时间：
2016-02-17
期刊：
Environmental health : a global access science source
影响因子：
--
作者：
Hooiveld M;Smit LAM;van der Sman-de Beer F;Wouters IM;van Dijk CE;Spreeuwenberg P;Heederik DJJ;Yzermans CJ
通讯作者：
Yzermans CJ
由同源二聚体 E2A-HLF 转录因子介导的细胞转化。
影响因子：
5.3
作者：
Inukai,T;Inaba,T;Yoshihara,T;Look,AT
通讯作者：
Look,AT