Stability and selectivity of unnatural DNA with five-membered-ring nucleobase analogues.
Stability and selectivity of unnatural DNA with five-membered-ring nucleobase analogues.
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具有五元环核碱基类似物的非天然 DNA 的稳定性和选择性。
DOI：
10.1021/ja012090t
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发表时间：
2002
影响因子：
15
通讯作者：
Romesberg,FloydE
中科院分区：
文献类型：
--
作者：
Berger,Markus;Luzzi,ScottD;Henry,AllisonA;Romesberg,FloydE
为了开发用于存储遗传信息的正交第三碱基对，噻吩和呋喃杂环已被检查为核碱基类似物。在相对于其他非天然碱基以及相对于天然碱基配对的双链体DNA中评价非天然碱基的稳定性。几个非天然的碱基对被鉴定为既合理稳定又对与天然碱基的错配具有强选择性。这些结果扩展了潜在的核碱基类似物，遗传字母表可以扩展到包括五元环杂环。
In an effort to develop an orthogonal third base pair for the storage of genetic information, thiophene and furan heterocycles have been examined as nucleobase analogues. The stability of the unnatural bases was evaluated in duplex DNA paired opposite other unnatural bases as well as opposite the natural bases. Several unnatural base pairs are identified that are both reasonably stable and strongly selective against mispairing with native bases. These results expand the potential nucleobase analogues with which the genetic alphabet may be expanded to include five-membered-ring heterocycles.