Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species
Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species
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DOI：
10.1095/biolreprod60.6.1496
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发表时间：
1999-06-01
影响因子：
3.6
通讯作者：
First, NL
中科院分区：
文献类型：
--
作者：
Dominko, T;Mitalipova, M;First, NL
细胞核从一个细胞到另一个细胞的转移为研究一个细胞的细胞质和另一个细胞的细胞核之间的相互作用提供了一个强大的工具。本研究旨在研究牛中期卵母细胞胞质在不同哺乳动物分化的体细胞核的指导下支持有丝分裂细胞周期的能力。来自牛、羊、猪、猴子和大鼠的皮肤成纤维细胞被用作供体核的来源。由去核的牛卵母细胞和来自所有物种的单个成纤维细胞融合产生的核移植单位经历了过渡到间期伴随着核肿胀，在细胞周期中进一步进展，并完成了第一次有丝分裂。无论供体成纤维细胞的种类如何，一些裂解单位进一步进展并发展到高级阶段，表现为细胞继续增殖并在适合供体成纤维细胞种类的时间形成囊胚腔。虽然胚胎移植到代孕动物体内后没有妊娠，但这些观察表明，调控早期胚胎发育的机制在哺乳动物物种中可能是保守的，牛卵母细胞细胞质可以支持引入的分化核，而无论捐赠者成纤维细胞的染色体数量、物种或年龄如何。
The transfer of nuclei from one cell to another provides a powerful tool for studying the interactions between the cytoplasm of one cell and the nucleus of another. This study was designed to examine the ability of the bovine metaphase oocyte cytoplasm to support mitotic cell cycles under the direction of differentiated somatic cell nuclei of various mammalian species. Skin fibroblast cells from cows, sheep, pigs, monkeys, and rats were used as sources of donor nuclei. Nuclear transfer units produced by fusion of enucleated bovine oocytes and individual fibroblasts from all species examined underwent transition to interphase accompanied by nuclear swelling, further progression through the cell cycle, and completion of the first mitosis. Regardless of the species of donor fibroblasts used, some cleaving units progressed further and developed to advanced stages, as evidenced by continuation of cell proliferation and formation of a blastocoele cavity at the time appropriate for the donor fibroblast species. Although no pregnancies have been carried to term after transfer of embryos into surrogate animals, these observations suggest that mechanisms regulating early embryonic development may be conserved among mammalian species and that bovine oocyte cytoplasm can support the introduced differentiated nucleus regardless of chromosome number, species, or age of the donor fibroblast.