High level of calpain1 promotes cancer cell invasion and migration in oral squamous cell carcinoma.
High level of calpain1 promotes cancer cell invasion and migration in oral squamous cell carcinoma.
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DOI：
10.3892/ol.2017.5970
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发表时间：
2017-06
期刊：
影响因子：
2.9
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Ma D;Fang J;Liu Y;Song JJ;Wang YQ;Xia J;Cheng B;Wang Z
钙蛋白酶1，称为常规钙蛋白酶和钙离子依赖性中性半胱氨酸蛋白酶的成员，被认为参与癌症的形成和发展。然而，钙蛋白酶1对口腔鳞状细胞癌（OSCC）的影响仍然知之甚少。本研究的目的是评估钙蛋白酶1作为口腔鳞癌诊断和治疗的潜在分子靶点的可能性。目前的研究表明，钙蛋白酶1过表达的OSCC细胞系和4/7的肿瘤组织配对样本的肿瘤和非癌匹配组织（NCMT）。在一个125例原发性口腔鳞癌患者的队列中，在多变量分析中，钙蛋白酶1的高表达是总生存率的独立预测因子（P=0.022）。此外，RNA干扰介导的抑制calpain 1表达降低了HSC 3和CAL 27 OSCC细胞系的增殖，迁移和侵袭潜力，但没有增加其凋亡。这些发现表明，钙蛋白酶1可能是一个有用的生物标志物，在口腔鳞状细胞癌的新的预后和治疗策略。
Calpain1, termed conventional calpain and a member of the Ca2+-dependent neutral cysteine proteases, is considered to be involved in cancer formation and development. However, the effect of calpain1 on oral squamous cell carcinoma (OSCC) remains poorly understood. The aim of the present study was to evaluate the possibility of calpain1 as a potential molecular target for OSCC diagnosis and therapy. The present study demonstrates that calpain1 was overexpressed in OSCC cell lines and 4/7 of the tumor tissues in paired samples of tumor and noncancerous matched tissues (NCMT). In a cohort of 125 patients with primary OSCC, the high expression of calpain1 was an independent predictor for overall survival in a multivariate analysis (P=0.022). Furthermore, RNA interference-mediated suppression of calpain1 expression reduced the proliferation, migration and invasion potential of the HSC3 and CAL27 OSCC cell lines, but did not increase their apoptosis. These findings indicate that calpain1 may be a useful biomarker for novel prognostic and therapeutic strategies in oral squamous cell carcinoma.