Expression of Fibroblast Activation Proteins in Corneal Stromal Neovascularization
Expression of Fibroblast Activation Proteins in Corneal Stromal Neovascularization
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成纤维细胞激活蛋白在角膜基质新生血管中的表达
DOI：
10.1080/02713680802607732
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发表时间：
2009-01
影响因子：
2
通讯作者：
Wang, Ting
中科院分区：
文献类型：
--
作者：
Shi, Weiyun;Wang, Ting
目的：观察角膜新生血管形成过程中角膜基质因子的变化，探讨角膜新生血管形成的机制。方法：选用Wister大鼠48只，其中对照组8只。碱烧伤诱导40只大鼠角膜新生血管形成。分别于烧伤后第1天、第3天和第7天在角膜中心处切开冷冻切片。免疫组织化学检测转化生长因子-β 1 (TGF-β 1)，双标记荧光免疫组织化学检测成纤维细胞活化蛋白（FAP）和a-平滑肌肌动蛋白（a-SMA）。在血管内皮中检测到PECAM-1 （CD31）。在第3天和第7天，用逆转录-聚合酶链反应（逆转录-聚合酶链反应）监测有或没有新生血管的角膜中FAP的表达。结果：在碱烧伤眼中，TGF-ß1首先在角膜基质中表达，部分基质细胞表达a-SMA和FAP。在血管生成的CD31+内皮细胞周围可见FAP+角化细胞。FAP在新生血管形成的角膜中表达，而在无新生血管形成的角膜中不表达。结论：角膜新生血管形成时，角膜基质因子可能发生改变。间质角化细胞可表达血管生成内皮周围的FAP+细胞。
Purpose: To observe changes of the factors in corneal stroma during corneal neovascularization and to investigate the mechanism of corneal neovascularization. Methods: Forty-eight Wister rats were used, including eight in the control group. Corneal neovascularization was induced by alkali burns in 40 rats. Frozen sections, which were cut across the corneal center, were prepared on days 1, 3, and 7 post-burn, respectively. Transforming growth factor-β 1 (TGF-β 1) was examined by immunohistochemistry, and fibroblast activation protein (FAP) and a-smooth muscle actin (a-SMA) were detected by double-labeling fluorescent immunohistochemistry. Blood vessel endothelium was identified for PECAM-1 (CD31). Expressions of FAP in the cornea with or without neovascularization were monitored with reverse transcription-polymerase chain reaction on days 3 and 7. Results: In the alkali-burned eyes, TGF-ß1 first expressed in the corneal stroma, and some stromal cells expressed a-SMA and FAP. The FAP+ keratocytes were found around the CD31+ endothelium of angiogenesis. FAP was expressed in the corneas with neovascularization, but not in those without neovascularization. Conclusion: Factors in corneal stroma may change when corneal neovascularization occurs. The stromal keratocytes can express FAP+ cells surrounding the endothelium of angiogenesis.
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影响因子：
11.2
作者：
Jonathan D. Cheng;Roland L. Dunbrack;Matthildi Valianou;A. Rogatko;R. Alpaugh;L. Weiner
通讯作者：
Jonathan D. Cheng;Roland L. Dunbrack;Matthildi Valianou;A. Rogatko;R. Alpaugh;L. Weiner
影响因子：
4.4
作者：
J. Jester;J. Jester;Jiying Huang;P. Barry-Lane;W. Kao;W. Petroll;H. D. Cavanagh
通讯作者：
J. Jester;J. Jester;Jiying Huang;P. Barry-Lane;W. Kao;W. Petroll;H. D. Cavanagh
影响因子：
5.3
作者：
Niedermeyer, J;Kriz, M;Schnapp, A
通讯作者：
Schnapp, A
DOI：
--
发表时间：
2003-05
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Jonathan D. Cheng;L. Weiner
通讯作者：
Jonathan D. Cheng;L. Weiner
影响因子：
6
作者：
Chambers, RC;Leoni, P;Heller, RA
通讯作者：
Heller, RA