ROLE OF bolA AND rpoS GENES IN BIOFILM FORMATION AND ADHERENCE PATTERN BY ESCHERICHIA COLI K-12 MG1655 ON POLYPROPYLENE, STAINLESS STEEL, AND SILICONE SURFACES
ROLE OF bolA AND rpoS GENES IN BIOFILM FORMATION AND ADHERENCE PATTERN BY ESCHERICHIA COLI K-12 MG1655 ON POLYPROPYLENE, STAINLESS STEEL, AND SILICONE SURFACES
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DOI：
10.1556/030.63.2016.018
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发表时间：
2017-06-01
影响因子：
1.5
通讯作者：
Hadi, Sibte
中科院分区：
文献类型：
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作者：
Adnan, Mohd;Sousa, Ana Margarida;Hadi, Sibte
大肠杆菌已经发展出复杂的手段来感知、响应和适应压力环境。自20世纪60年代以来，它一直是分子遗传学和生理学研究的模式生物。每当细胞需要适应并在不利的生长条件下生存时，就会诱导应激反应基因。两个可能的重要基因是rpoS和bolA。rpoS基因被称为替代西格玛（sigma）因子，其控制大量基因的表达，这些基因参与对各种应激因子的响应以及从指数形式的生长过渡到稳定期。形态基因bolA对胁迫环境的响应导致E.大肠杆菌细胞，但很少有人知道它参与生物膜及其发展或维护。本研究旨在探讨大肠杆菌的粘附模式和生物膜的形成。大肠杆菌在37 ℃下生长24小时后，在不锈钢、聚丙烯和硅酮表面上。扫描电子显微镜用于直接检查细胞附着和生物膜形成的各种表面上，它被发现，在存在bolA，E。大肠杆菌细胞能够很好地附着在不锈钢和硅胶上。相比之下，聚丙烯表面对大肠杆菌没有吸引力。coli细胞。这表明，bolA的反应，并可以发挥重要作用的存在和不存在的rpoS在细胞附着。
Escherichia coli has developed sophisticated means to sense, respond, and adapt in stressed environment. It has served as a model organism for studies in molecular genetics and physiology since the 1960s. Stress response genes are induced whenever a cell needs to adapt and survive under unfavorable growth conditions. Two of the possible important genes are rpoS and bolA. The rpoS gene has been known as the alternative sigma (sigma) factor, which controls the expression of a large number of genes, which are involved in responses to various stress factors as well as transition to stationary phase from exponential form of growth. Morphogene bolA response to stressed environment leads to round morphology of E. coli cells, but little is known about its involvement in biofilms and its development or maintenance. This study has been undertaken to address the adherence pattern and formation of biofilms by E. coli on stainless steel, polypropylene, and silicone surfaces after 24 h of growth at 37 degrees C. Scanning electron microscopy was used for direct examination of the cell attachment and biofilm formation on various surfaces and it was found that, in the presence of bolA, E. coli cells were able to attach to the stainless steel and silicone very well. By contrast, polypropylene surface was not found to be attractive for E. coli cells. This indicates that bolA responded and can play a major role in the presence and absence of rpoS in cell attachment.