GLOBAL CHANGES IN PROTEIN-SYNTHESIS DURING ADAPTATION OF THE YEAST SACCHAROMYCES-CEREVISIAE TO 0.7 M NACL
GLOBAL CHANGES IN PROTEIN-SYNTHESIS DURING ADAPTATION OF THE YEAST SACCHAROMYCES-CEREVISIAE TO 0.7 M NACL
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DOI：
10.1128/jb.177.12.3563-3572.1995
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发表时间：
1995-06-01
影响因子：
3.2
通讯作者：
BLOMBERG, A
中科院分区：
文献类型：
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作者：
BLOMBERG, A
通过转移到 0.7 M NaCl 培养基中，指数生长的酿酒酵母面临盐度增加的挑战，并通过使用二维凝胶电泳与计算机定量相结合，在适应的第一小时内检查蛋白质合成的变化。大量蛋白质显示出相对合成速率的变化，与非应激细胞的大多数差异出现在 20 至 40 分钟之间。在此期间，18 种蛋白质的合成率增加了八倍以上，并被归类为高度 NaCl 响应性蛋白质。只有两种蛋白质被抑制到相同的水平。大多数这些高度 NaCl 响应性的蛋白质似乎构成了先前未报道过的对脱水作出响应的基因产物。将选择标准应用于相对合成速率的两倍变化的后续样本，识别出 14 种不同的调控模式。大多数已鉴定的糖酵解酶表现出延迟反应，它们的合成速率直到适应中期才发生变化，仅产生速率略有下降。 HSP70 基因家族的一些成员观察到轻微的盐刺激反应。总体而言，所提供的数据表明在适应 NaCl 期间存在复杂的细胞内信号传导以及多种调节机制的参与。
Exponentially growing Saccharomyces cerevisiae was challenged to increased salinity by transfer to 0.7 M NaCl medium, and changes in protein synthesis were examined during the 1st h of adaptation by use of two-dimensional gel electrophoresis coupled to computerized quantification. An impressive number of proteins displayed changes in the relative rate of synthesis, with most differences from nonstressed cells being found at between 20 and 40 min. During this period, 18 proteins exhibited more than eightfold increases in their rates of synthesis and were classified as highly NaCl responsive. Only two proteins were repressed to the same level. Most of these highly NaCl-responsive proteins seemed to constitute gene products not earlier reported to respond to dehydration, Applying a selection criterion to subsequent samples of a twofold change in the relative rate of synthesis, 14 different regulatory patterns were discerned. Most identified glycolytic enzymes exhibited a delayed response, and their rates of synthesis did not change until the middle phase of adaptation, with only a minor decrease in the rate of production. A slight salt-stimulated response was observed for some members of the HSP70 gene family. Overall, the data presented indicate complex intracellular signalling as well as involvement of diverse regulatory mechanisms during the period of adaptation to NaCl.