Validation and Characterization of Ghd7.1, a Major Quantitative Trait Locus with Pleiotropic Effects on Spikelets per Panicle, Plant Height, and Heading Date in Rice (Oryza sativa L.)
Validation and Characterization of Ghd7.1, a Major Quantitative Trait Locus with Pleiotropic Effects on Spikelets per Panicle, Plant Height, and Heading Date in Rice (Oryza sativa L.)
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DOI：
10.1111/jipb.12070
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发表时间：
2013-10-01
影响因子：
11.4
通讯作者：
Xing, Yongzhong
中科院分区：
文献类型：
--
作者：
Liu, Touming;Liu, Haiyang;Xing, Yongzhong
水稻（Oryza sativa L.）在7号染色体上的一个小区域发现了一个影响抽穗日期（HD）和每穗小粒数的数量性状位点（QTL）。为了进一步对QTL区域进行表征，利用在目标区域附近杂合的重组自交系189进行自交，快速获得了近等基因系（NILs）。利用NIL-F-2群体验证了QTL Ghd7.1对株高（PH）、SPP和HD的多效效应。Ghd7.1分别解释了PH、SPP和HD的50.2%、45.3%和76.9%的表型变异。在分析nilf -2群体后代的基础上，将Ghd7.1精确定位到357 kb的区域。日长处理证实了Ghd7.1对光周期敏感，延迟抽穗时间长达12.5 d。对NILs的穗萌发发育鉴定表明，Ghd7.1将光周期敏感期延长了10 d以上，但没有改变基本营养期和生殖生长期。这些结果表明，Ghd7.1通过控制穗分化速率而不是穗发育时间来调控SPP。
A quantitative trait locus (QTL) that affects heading date (HD) and the number of spikelets per panicle (SPP) was previously identified in a small region on chromosome 7 in rice (Oryza sativa L.). In order to further characterize the QTL region, near isogenic lines (NILs) were quickly obtained by self-crossing recombinant inbred line 189, which is heterozygous in the vicinity of the target region. The pleiotropic effects of QTL Ghd7.1 on plant height (PH), SPP, and HD, were validated using an NIL-F-2 population. Ghd7.1 explained 50.2%, 45.3%, and 76.9% of phenotypic variation in PH, SPP, and HD, respectively. Ghd7.1 was precisely mapped to a 357-kb region on the basis of analysis of the progeny of the NIL-F-2 population. Day-length treatment confirmed that Ghd7.1 is sensitive to photoperiod, with long days delaying heading up to 12.5 d. Identification of panicle initiation and development for the pair of NILs showed that Ghd7.1 elongated the photoperiod-sensitive phase more than 10 d, but did not change the basic vegetative phase and the reproductive growth phase. These findings indicated that Ghd7.1 regulates SPP by controlling the rate of panicle differentiation rather than the duration of panicle development.