Internal derivatization of oligonucleotides with selenium for X-ray crystallography using MAD
Internal derivatization of oligonucleotides with selenium for X-ray crystallography using MAD
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DOI：
10.1021/ja0171097
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发表时间：
2002-01-09
影响因子：
15
通讯作者：
Huang, Z
中科院分区：
文献类型：
--
作者：
Du, Q;Carrasco, N;Huang, Z
我们已经开发了一种合成2 '-硒尿苷类似物和含硒标记的寡核苷酸的路线，并首次证明了一种新的策略，用硒共价衍生核苷酸，用于X射线晶体学中的相和结构测定。
We have developed a route for the synthesis of 2‘-selenium uridine analogues and oligonucleotides containing selenium labels, and have demonstrated for the first time a new strategy to covalently derivatize nucleotides with selenium for phase and structure determination in X-ray crystallography.