Angiopoietin-2 is increased in severe sepsis: Correlation with inflammatory mediators
Angiopoietin-2 is increased in severe sepsis: Correlation with inflammatory mediators
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DOI：
10.1097/01.ccm.0000251640.77679.d7
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发表时间：
2007-01-01
影响因子：
8.8
通讯作者：
Roussos, Charis
中科院分区：
文献类型：
--
作者：
Orfanos, Stylianos E.;Kotanidou, Anastasia;Roussos, Charis
目的：血管生成素（Ang）-2是一种内皮特异性生长因子，受促炎刺激调节，使血管内皮不稳定并增加血管渗漏;因此，Ang-2可能有助于脓毒症的病理生理学。我们研究了1）危重病患者血清Ang-2水平，并研究了与炎症介质和疾病严重程度指数的潜在关系，以及2）脓毒症相关炎症介质对体外肺内皮细胞产生Ang-2的影响。前瞻性临床研究，随后进行细胞培养研究。一所大学医院的普通重症监护室和研究实验室。受试者：人和牛肺微血管内皮细胞和61例患者（32例男性）。根据脓毒症分期将患者分组为：无全身炎症反应综合征（n = 6）、全身炎症反应综合征（n = 8）、脓毒症（n = 16）、严重脓毒症（n = 18）和脓毒性休克（n = 13）。将细胞暴露于脂多糖、肿瘤坏死因子-α和白细胞介素-6。与无全身炎症反应综合征或脓毒症患者相比，严重脓毒症患者血清Ang-2水平显著升高（p
Objective: Angiopoietin (Ang)-2 is an endothelium-specific growth factor, regulated by proinflammatory stimuli, that destabilizes vascular endothelium and increases vascular leakage; consequently, Ang-2 may contribute to sepsis pathophysiology. We have studied 1) serum Ang-2 levels in critically-ill patients and investigated potential relationships with inflammatory mediators and indices of disease severity and 2) the effect of sepsis-related inflammatory mediators on Ang-2 production by lung endothelium in vitro.Design. Prospective clinical study followed by cell culture studies.Setting. General intensive care unit and research laboratory of a university hospital. Subjects: Human and bovine lung microvascular endothelial cells and 61 patients (32 men). Patients were grouped according to their septic stage as having: no systemic inflammatory response syndrome (n = 6), systemic inflammatory response syndrome (n = 8), sepsis (n = 16), severe sepsis (n = 18), and septic shock (n = 13).Interventions. Cells were exposed to lipopolysaccharide, tumor necrosis factor-alpha, and interleukin-6.Measurements and Main Results., Patients' serum Ang-2 levels were significantly increased in severe sepsis as compared with patients with no systemic inflammatory response syndrome or sepsis (p