A macrophage Fc receptor for IgG is also a receptor for oxidized low density lipoprotein.
A macrophage Fc receptor for IgG is also a receptor for oxidized low density lipoprotein.
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DOI：
10.1016/s0021-9258(18)41692-4
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发表时间：
1992-11
期刊：
影响因子：
--
通讯作者：
Lawrence W. Stanton;R. T. White;C. M. Bryant;Andrew A. Protter;G. Endemann
中科院分区：
文献类型：
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作者：
Lawrence W. Stanton;R. T. White;C. M. Bryant;Andrew A. Protter;G. Endemann
氧化型低密度脂蛋白（OxLDL）的巨噬细胞的内化被假设为有助于泡沫细胞的形成，并最终动脉粥样硬化病变的形成。OxLDL是乙酰化低密度脂蛋白（AcLDL）受体的配体，然而，我们的数据显示，该受体占小鼠巨噬细胞摄取OxLDL的不到一半，表明OxLDL的其他受体。我们已经开发了一种新的表达克隆策略，以分离编码OxLDL受体的克隆。除了AcLDL受体，我们分离出一个分子克隆的结构无关的受体能够介导高亲和力摄取OxLDL转染细胞后。该受体已被鉴定为小鼠Fc γ RII-B2，是已知通过识别IgG的Fc区介导免疫复合物摄取的受体家族的成员。用Fc γ RII-B2克隆转染的细胞对OxLDL的摄取未被AcLDL阻断，但被抗Fc γ RII单克隆抗体2.4G2阻断。
The internalization of oxidized low density lipoprotein (OxLDL) by macrophages is hypothesized to contribute to foam cell formation and eventually to atherosclerotic lesion formation. OxLDL is a ligand for the acetylated low density lipoprotein (AcLDL) receptor, however, our data show that this receptor accounts for less than half of OxLDL uptake by mouse macrophages, suggesting additional receptors for OxLDL. We have developed a novel expression cloning strategy in order to isolate clones encoding OxLDL receptors. In addition to the AcLDL receptor, we isolated a molecular clone for a structurally unrelated receptor capable of mediating the high affinity uptake of OxLDL following transfection into cells. This receptor has been identified as the mouse Fc gamma RII-B2, a member of a family of receptors known to mediate immune complex uptake through recognition of the Fc region of IgG. The uptake of OxLDL by cells transfected with the Fc gamma RII-B2 clone is not blocked by AcLDL but is blocked by the anti-Fc gamma RII monoclonal antibody, 2.4G2.