Epitranscriptomic profiling of N4-acetylcytidine-related RNA acetylation in the spinal dorsal horn of rat with cancer-induced bone pain.
Epitranscriptomic profiling of N4-acetylcytidine-related RNA acetylation in the spinal dorsal horn of rat with cancer-induced bone pain.
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DOI：
10.1177/17448069231178487
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发表时间：
2023-01
期刊：
影响因子：
3.3
通讯作者：
中科院分区：
文献类型：
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作者：
近年来，表观遗传学参与基因表达调控已成为研究热点。本研究评估了癌症诱发骨痛（CIBP）大鼠脊髓背角（SDH）中N4-乙酰胞苷（ac 4c）RNA乙酰化。采用ac 4C特异性RIP测序和NAT 10特异性RIP测序方法，比较CIBP组和假手术组SDH中ac 4C乙酰化和基因表达的差异，以及与乙酰化修饰酶NAT 10的关系，并进行关联分析。通过干扰NAT 10的表达，验证了CIBP中一些上调基因与ac 4C乙酰化的关系。在这项研究中，我们证明了骨癌增加了NAT 10和整体乙酰化的水平，诱导大鼠SDH中的不同ac 4C模式。通过验证实验发现，某些基因的ac 4C乙酰化受NAT 10调控，RNA中的差异ac 4C模式决定了该RNA的表达。我们发现，一些CIBP相关基因的表达改变，在SDH的大鼠，这是调节差异表达ac 4C乙酰化。
Recently, epigenetics involved in the regulation of gene expression has become a research hotspot. This study evaluated N4-acetylcytidine (ac4c) RNA acetylation in the spinal dorsal horn (SDH) of rats with cancer-induced bone pain (CIBP). The ac4C-specific RIP sequencing and NAT10-specific RIP sequencing were performed to identify the differences in ac4C acetylation and gene expression in the SDH between CIBP and sham groups, the relationship with the acetylation-modifying enzyme NAT10, and association analysis was performed. By interfering with the NAT10 expression, the relationship between some up-regulated genes and ac4C acetylation in CIBP was verified. In this study, we demonstrated that bone cancer increases the levels of NAT10 and the overall acetylation, inducing differential ac4C patterns in the SDH of rats. Through verification experiments, it was found that ac4C acetylation of some genes is regulated by NAT10, and differential ac4C patterns in RNA determine the expression of this RNA. We exposed that some CIBP-related gene expression was altered in the SDH of rats, which was regulated by differentially expressed ac4C acetylation.
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影响因子：
14.9
作者：
Hauenschild R;Tserovski L;Schmid K;Thüring K;Winz ML;Sharma S;Entian KD;Wacheul L;Lafontaine DL;Anderson J;Alfonzo J;Hildebrandt A;Jäschke A;Motorin Y;Helm M
通讯作者：
Helm M
影响因子：
14.9
作者：
Boccaletto P;Stefaniak F;Ray A;Cappannini A;Mukherjee S;Purta E;Kurkowska M;Shirvanizadeh N;Destefanis E;Groza P;Avşar G;Romitelli A;Pir P;Dassi E;Conticello SG;Aguilo F;Bujnicki JM
通讯作者：
Bujnicki JM
影响因子：
5
作者：
Xu, Longsheng;Wang, Shizhen;Yao, Ming
通讯作者：
Yao, Ming
5-甲基胞嘧啶促进 mRNA 输出-NSUN2 作为甲基转移酶，ALYREF 作为 m5C 阅读器
影响因子：
44.1
作者：
Yang X;Yang Y;Sun BF;Chen YS;Xu JW;Lai WY;Li A;Wang X;Bhattarai DP;Xiao W;Sun HY;Zhu Q;Ma HL;Adhikari S;Sun M;Hao YJ;Zhang B;Huang CM;Huang N;Jiang GB;Zhao YL;Wang HL;Sun YP;Yang YG
通讯作者：
Yang YG
DOI：
10.7759/cureus.30456
发表时间：
2022-10
期刊：
Cureus
影响因子：
--
作者：
Khalil DT;Slater K;Cooper C
通讯作者：
Cooper C