Tissue-specific gene silencing by RNA interference in the whitefly Bemisia tabaci (Gennadius)
Tissue-specific gene silencing by RNA interference in the whitefly Bemisia tabaci (Gennadius)
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DOI：
10.1016/j.ibmb.2007.04.006
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发表时间：
2007-07-01
影响因子：
3.8
通讯作者：
Czosnek, Henryk
中科院分区：
文献类型：
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作者：
Ghanim, Murad;Kontsedalov, Svetlana;Czosnek, Henryk
半翅目烟粉虱Bemisia tabaci（Gennadius）物种复合体和它们传播的植物病毒对全世界的蔬菜和纤维生产构成主要限制。粉虱的组织和发育特异性基因表达尚未详尽的研究，尽管其经济的重要性。2002年启动了一个功能基因组计划，产生了数千个表达序列标签（EST）及其序列。该项目为设计旨在理解和操纵粉虱基因表达的实验提供了基本信息。在这次交流中，我们首次提供证据表明，在许多生物体中发现的RNA干扰机制，包括半翅目，在B中是活跃的。烟粉虱通过向体腔内注射长双链RNA（dsRNA）分子，特异性地针对中肠和唾液腺中独特表达的基因，我们能够显著抑制靶向mRNA在不同器官中的表达。与注射缓冲液或绿色荧光蛋白（GFP）特异性dsRNA的粉虱相比，RNAi沉默的粉虱中的基因表达水平降低高达70%。在B中观察到表型效应。烟粉虱卵巢后靶向果蝇山雀同源物的dsRNA。粉虱基因表达的破坏为旨在遏制这种昆虫害虫对农业的有害影响的新策略打开了大门。(c)2007爱思唯尔有限公司版权所有。
The hemipteran whitefly Bemisia tabaci (Gennadius) species complex and the plant viruses they transmit pose major constraints to vegetable and fiber production, worldwide. The whitefly tissue- and developmental-specific gene expression has not been exhaustively studied despite its economic importance. In 2002, a functional genomic project was initiated, which generated several thousands expressed sequence tags (ESTs) and their sequence. This project provides the basic information to design experiments aimed at understanding and manipulating whitefly gene expression. In this communication, for the first time we provide evidence that the RNA interference mechanism discovered in many organisms, including in Hemiptera, is active in B. tabaci. By injecting into the body cavity long double-stranded RNA (dsRNA) molecules, specifically directed against genes uniquely expressed in the midgut and salivary glands, we were able to significantly inhibit the expression of the targeted mRNA in the different organs. Gene expression levels in RNAi-silenced whiteflies were reduced up to 70% compared to whiteflies injected with buffer or with a green fluorescent protein (GFP)-specific dsRNA. Phenotypic effects were observed in B. tabaci ovaries following dsRNA targeting the whitefly Drosophila chickadee homologue. Disruption of whitefly gene expression opens the door to new strategies aimed at curbing down the deleterious effects of this insect pest to agriculture. (c) 2007 Elsevier Ltd. All rights reserved.