Controlled Growth Factor Release in 3D‐Printed Hydrogels
Controlled Growth Factor Release in 3D‐Printed Hydrogels
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DOI：
10.1002/adhm.201900977
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发表时间：
2019-11
影响因子：
10
通讯作者：
Pengrui Wang;D. Berry;A. Moran;F. He;Trevor Tam;Luwen Chen;Shaochen Chen
中科院分区：
文献类型：
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作者：
Pengrui Wang;D. Berry;A. Moran;F. He;Trevor Tam;Luwen Chen;Shaochen Chen
生长因子（GF）是组织再生过程中控制细胞命运的关键组成部分，这是由于体内快速的转离速率，例如基于肝素的水凝胶已经研究了各种浓度和分子量的保留效应，几何作用在这项工作中尚不清楚。用任意复杂的几何形状（即茶袋，花形）制造的基于GF的基于肝素的水凝胶壳层的厚度降低了GF释放的速率。壳层大于0.5毫米（R2> 0.96），通过切换空间层来证明顺序释放如何利用3D打印来制造具有独特几何形状的用户定义结构，以控制水凝胶中的GF释放速率。
Growth factors (GFs) are critical components in governing cell fate during tissue regeneration. Their controlled delivery is challenging due to rapid turnover rates in vivo. Functionalized hydrogels, such as heparin‐based hydrogels, have demonstrated great potential in regulating GF release. While the retention effects of various concentrations and molecular weights of heparin have been investigated, the role of geometry is unknown. In this work, 3D printing is used to fabricate GF‐embedded heparin‐based hydrogels with arbitrarily complex geometry (i.e., teabag, flower shapes). Simplified cylindrical core–shell structures with varied shell thickness are printed, and the rates of GF release are measured over the course of 28 days. Increasing the shell layers' thickness decreases the rate of GF release. Additionally, a mathematical model is developed, which is found capable of accurately predicting GF release kinetics in hydrogels with shell layers greater than 0.5 mm thick (R2 > 0.96). Finally, the sequential release is demonstrated by printing two GFs in alternating radial layers. By switching the spatial order, the delivery sequence of the GFs can be modulated. This study demonstrates how 3D printing can be utilized to fabricate user‐defined structures with unique geometry in order to control the rate of GF release in hydrogels.