REDUCED SENSITIVITY OF HYPOTHALAMIC-PREOPTIC AREA NOREPINEPHRINE AND DOPAMINE TO TESTOSTERONE FEEDBACK IN ADULT FETAL ETHANOL-EXPOSED MALE-RATS
REDUCED SENSITIVITY OF HYPOTHALAMIC-PREOPTIC AREA NOREPINEPHRINE AND DOPAMINE TO TESTOSTERONE FEEDBACK IN ADULT FETAL ETHANOL-EXPOSED MALE-RATS
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DOI：
10.1016/0741-8329(90)90041-a
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发表时间：
1990-11-01
期刊：
影响因子：
2.3
通讯作者：
RUDEEN, PK
中科院分区：
文献类型：
--
作者：
JUNGKUNTZBURGETT, L;PAREDEZ, S;RUDEEN, PK
睾酮（T）在下丘脑调节中的内分泌反馈是通过T对去甲肾上腺素能系统的作用实现的。本实验观察了胎儿期乙醇暴露（FEE）对成年雄性大鼠下丘脑视前区（HPOA）去甲肾上腺素（NE）和多巴胺（DA）含量的影响，以及NE和DA对T的反应。将妊娠大鼠暴露于含乙醇的液体饮食、含蔗糖等热量替代乙醇的液体饮食或食物和水饮食的饮食。雄性后代在45日龄时去势或假手术。动物接受丙酸睾酮（TP）或油溶剂。在55日龄时从每只动物收集HPOA，并通过HPLC-EC测量NE和DA含量。有没有显着的变化，NE或DA含量在HPOA在FEE动物相比，动物中的儿茶酚胺水平来自母兽的控制饮食。 去势对饲料喂养或配对喂养动物的NE和DA含量无显著影响。TP管理显着降低NE含量仅在饲料和配对喂养的动物，但不是在FEE动物。在HPOA中的DA含量不受去势的影响，但TP管理也导致显着降低DA含量的饲料和配对喂养的去势雄性大鼠，但在不FEE去势雄性大鼠。结果表明，FEE改变了HPOA的去甲肾上腺素能和多巴胺能神经元对T给药的反应。给予高剂量TP的去势大鼠中HPOA、NE和DA含量的抑制在暴露于乙醇的胎鼠中显著减少，这表明神经元对下丘脑类固醇反馈调节的反应性降低。
Endocrine feedback of testosterone (T) in the regulation of the hypothalamus is via the effects of T on the noradrenergic system. The current experiment was performed to determine the effects of fetal ethanol exposure (FEE) on the norepinephrine (NE) and dopamine (DA) content in the hypothalamic -preoptic area (HPOA) of adult male rats, and the response of NE and DA to T administration. Pregnant rats were exposed to diets containing either a liquid diet containing ethanol, a liquid diet containing sucrose isocalorically substituted for ethanol, or a chow and water diet. Male offspring were castrated or sham-operated at 45 days of age. The animals received either testosterone propionate (TP) or an oil vehicle. HPOA was collected at 55 days of age from each animal and NE and DA content was measured by HPLC-EC. There was no significant alteration of NE or DA content in the HPOA in FEE animals compared to catecholamine levels in animals derived from dams on the control diets. Castration had no significant effect on NE and DA content in the chow-fed or pair-fed animals. TP administration significantly reduced NE content only in the chow- and pair-fed animals but not in the FEE animals. DA content in the HPOA was not affected by castration, but TP administration also resulted in significantly reducing DA content in chow- and pair-fed castrate male rats but in not FEE castrate male rats. The results indicate that FEE alters the response of the noradrenergic and dopaminergic neurons in the HPOA to T administration. The inhibition of HPOA NE and DA content in castrated rats given high doses of TP is significantly diminished in fetal ethanol-exposed rats that is indicative of a reduction of neuronal responsiveness to steroid feedback regulation of the hypothalamus.