RET expression in papillary thyroid cancer from patients irradiated in childhood for benign conditions.
RET expression in papillary thyroid cancer from patients irradiated in childhood for benign conditions.
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DOI：
10.1210/jcem.87.8.8748
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发表时间：
2002-08
期刊：
影响因子：
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通讯作者：
B. J. Collins;G. Chiappetta;A. Schneider;M. Santoro;F. Pentimalli;L. Fogelfeld;T. Gierlowski;E. Shore-Freedman;G. Jaffe;A. Fusco
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文献类型：
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作者：
B. J. Collins;G. Chiappetta;A. Schneider;M. Santoro;F. Pentimalli;L. Fogelfeld;T. Gierlowski;E. Shore-Freedman;G. Jaffe;A. Fusco
外部和内部的辐射暴露都与甲状腺乳头状癌的发生有关。 RET 酪氨酸激酶基因的重排和随后该蛋白的表达也被发现发生在许多乳头状甲状腺癌中，并且在切尔诺贝利事故后与辐射相关的癌症中发生频率增加。然而，关于暴露于外部辐射后癌症中 RET 重排频率的报道很少。我们在这里报告石蜡包埋甲状腺样本中的 RET 蛋白免疫反应性，这些样本来自 30 名乳头状甲状腺癌患者，这些患者在童年时期因良性病症在芝加哥 Michael Reese 医院接受过放射治疗，以及 34 名从肿瘤登记处确定患有乳头状甲状腺癌且没有放射治疗史的患者。受试者的特征包括性别、手术年龄以及肿瘤病理学的以下属性：大小、涉及的肺叶数量、病灶数量、淋巴结转移和软组织侵犯。代表性组织样本与针对 RET 酪氨酸激酶结构域的抗体发生反应，该结构域的表达已被证明与 RET/PTC 重排高度相关。在辐射暴露组中发现 RET 免疫反应呈阳性的癌症比例较高（86.7% vs. 52.9%，P = 0.006）。尽管暴露组的平均手术年龄低于对照组，但RET免疫反应阳性与手术年龄没有相关性。肿瘤特征与 RET 免疫反应阳性无关。总之，辐射组中RET免疫阳性的发生率较高，表明RET免疫反应性的表达与辐射暴露密切相关，但其预后意义尚不清楚。
Both external and internal exposure to radiation have been linked to the development of papillary thyroid cancer. Rearrangement of the gene for RET tyrosine kinase and subsequent expression of this protein has also been found to occur in many papillary thyroid cancers, and with increased frequency in radiation-related cancers following the Chernobyl accident. However, little has been reported on the frequency of RET rearrangements in cancers after exposure to external radiation. We here report on RET protein immunoreactivity in paraffin-embedded thyroid samples from 30 patients with papillary thyroid cancer who received radiation treatment during childhood for benign conditions at Michael Reese Hospital in Chicago, and in 34 patients identified from the tumor registry as having papillary thyroid cancer with no history of therapeutic radiation. The subjects were characterized by sex, age at surgery, and the following attributes of tumor pathology: size, number of lobes involved, number of foci, lymph node metastases, and soft tissue invasion. Representative tissue samples were reacted with an antibody against the RET tyrosine kinase domain whose expression has been shown to correlate highly with RET/PTC rearrangements. A greater percentage of cancers positive for RET immunoreactivity was found in the radiation-exposed group (86.7% vs. 52.9%, P = 0.006). Although the mean age at surgery of the exposed group was lower than the control group, there was no correlation of positive RET immunoreactivity with the age at surgery. No characteristics of the tumors were associated with positive RET immunoreactivity. In summary, the greater incidence of RET-immunopositives in the irradiated group indicates that the expression of RET immunoreactivity is strongly associated with radiation exposure, but the prognostic significance of this is not yet clear.