Microsatellite markers reveal population genetic structure of the toxic dinoflagellate Alexandrium tamarense (Dinophyceae) in Japanese coastal waters
Microsatellite markers reveal population genetic structure of the toxic dinoflagellate Alexandrium tamarense (Dinophyceae) in Japanese coastal waters
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DOI：
10.1111/j.1529-8817.2006.00304.x
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发表时间：
2007-02-01
影响因子：
2.9
通讯作者：
Hogetsu, Taizo
中科院分区：
文献类型：
--
作者：
Nagai, Satoshi;Lian, Chunlan;Hogetsu, Taizo
这是第一份报告，探讨日本和韩国沿海沃茨的一个典型的浮游微生物的精细尺度多样性，种群遗传结构，并试图检测自然和人为辅助扩散的遗传结构和基因流的影响，在有毒甲藻物种。在这里，我们提出了来自日本和韩国沿海沿着10个地点的塔玛亚历山大（Leopalum）Balech种群的遗传分析。我们使用了9个微卫星位点，这些位点在不同人群中的等位基因数量和基因多样性差异很大。分析表明，在成对比较中，Nei氏遗传距离与地理距离显著相关，45个成对群体中约有一半存在遗传分化。这些结果清楚地表明，根据地理距离和有限的基因流通过自然扩散，通过潮流之间的种群遗传隔离。另一方面，高P值的Fisher联合检验中检测到5个成对的群体，表明相似的遗传结构和密切的遗传关系之间的群体。这些结果表明，日本A。tamarense种群受到干扰，可能是由于人类协助的扩散，导致地理上分离的种群之间的基因流动。
This is the first report to explore the fine-scale diversity, population genetic structure, and biogeography of a typical planktonic microbe in Japanese and Korean coastal waters and also to try to detect the impact of natural and human-assisted dispersals on the genetic structure and gene flow in a toxic dinoflagellate species. Here we present the genetic analysis of Alexandrium tamarense (Lebour) Balech populations from 10 sites along the Japanese and Korean coasts. We used nine microsatellite loci, which varied widely in number of alleles and gene diversity across populations. The analysis revealed that Nei's genetic distance correlated significantly with geographic distance in pair-wise comparisons, and that there was genetic differentiation in about half of 45 pair-wise populations. These results clearly indicate genetic isolation among populations according to geographic distance and restricted gene flow via natural dispersal through tidal currents among the populations. On the other hand, high P-values in Fisher's combined test were detected in five pair-wise populations, suggesting similar genetic structure and a close genetic relationship between the populations. These findings suggest that the genetic structure of Japanese A. tamarense populations has been disturbed, possibly by human-assisted dispersal, which has resulted in gene flow between geographically separated populations.