A plant peptide encoded by CLV3 identified by in situ MALDI-TOF MS analysis
A plant peptide encoded by CLV3 identified by in situ MALDI-TOF MS analysis
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DOI：
10.1126/science.1128439
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发表时间：
2006-08-11
期刊：
影响因子：
56.9
通讯作者：
Sakagami, Youji
中科院分区：
文献类型：
--
作者：
Kondo, Tatsuhiko;Sawa, Shinichiro;Sakagami, Youji
拟南芥CLAVATA 3（CLV 3）基因编码干细胞特异性蛋白质，推测其为分泌肽激素的前体。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）应用于原位拟南芥组织确定了一个修饰的12个氨基酸的肽（MCLV 3），这是来自CLV 3序列中的保守基序的结构。合成的MCLV 3诱导芽和根分生组织的消耗，细胞分化成其他器官，表现出典型的过表达CLV 3的转基因植物的表型。这些结果表明，CLV 3的功能肽是MCLV 3。
The Arabidopsis CLAVATA3 (CLV3) gene encodes a stem cell - specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ Arabidopsis tissues determined the structure of a modified 12 - amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing CLV3. These results suggest that the functional peptide of CLV3 is MCLV3.