Gas-filled microbubbles: Novel mucosal antigen-delivery system for induction of anti-pathogen's immune responses in the gut
Gas-filled microbubbles: Novel mucosal antigen-delivery system for induction of anti-pathogen's immune responses in the gut
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DOI：
10.1080/19490976.2017.1334032
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发表时间：
2017-01-01
期刊：
影响因子：
12.2
通讯作者：
Bioley, Gilles
中科院分区：
文献类型：
--
作者：
Corthesy, Blaise;Bioley, Gilles
尽管在保护个体免受许多病原性感染方面取得了重要成功，但肠胃外疫苗接种对于在病原体进入部位（即粘膜表面）诱导免疫不是最佳的。此外，设计适当的递送系统和安全的佐剂以克服粘膜组织的固有致耐受性环境是具有挑战性的，特别是在易于抗原降解的胃肠道中。我们最近证明，与充气微泡相关的沙门氏菌衍生抗原的鼻内给药在肠道中诱导特异性Ab和T细胞应答，并且与口服沙门氏菌感染后局部和全身细菌负荷的减少相关。在这些有希望的数据的基础上，讨论了要施用的抗原的适当选择以及如何使其适合于可能的人类应用。此外，我们提出了新的数据处理与口服给药的微泡和描述的研究策略，将它们引导到粘膜采样/诱导网站。
Despite important success in protecting individuals against many pathogenic infections, parenteral vaccination is not optimal to induce immunity at the site of pathogen entry, i.e. mucosal surfaces. Moreover, designing adequate delivery systems and safe adjuvants to overcome the inherent tolerogenic environment of the mucosal tissue is challenging, in particular in the gastrointestinal tract prone to antigen degradation. We recently demonstrated that intranasal administration of a Salmonella-derived antigen associated with gas-filled microbubbles induced specific Ab and T cell responses in the gut and was associated with a reduction in local and systemic bacterial load after oral Salmonella infection. Building on these promising data, the adequate choice of antigen(s) to be administered and how to make it suitable for possible human application are discussed. We additionally present novel data dealing with oral administration of microbubbles and describe research strategies to direct them to mucosal sampling/inductive sites.