RNA induced reversal of glucocorticoid receptor activation.
RNA induced reversal of glucocorticoid receptor activation.
复制标题
RNA诱导糖皮质激素受体激活的逆转。
DOI：
10.1016/s0006-291x(82)80005-3
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
Hawkins,EF
中科院分区：
文献类型：
--
作者：
Hutchens,TW;Markland,FS;Hawkins,EF
部分纯化、活化(核结合)的乳腺糖皮质激素受体与胞浆中存在的大分子结合，将该受体转化为具有天然受体特性的形式。天然受体经盐激活和DEAE-纤维素层析纯化后，在4.0s处以蔗糖密度梯度沉淀，并结合(80-90%)ATP-琼脂糖凝胶。这种部分纯化的、激活的受体与粗胞浆中存在的一个因子结合，使受体沉积在天然受体的位置(8.4S)。此外，这种受体形式，像天然受体一样，不与ATPagose显著相互作用。负责这种受体转换的胞浆因子是大分子的(MW>；5000)，不与ATP-琼脂糖凝胶相互作用。在8M尿素/1%十二烷基硫酸钠/1 mM DTT存在和不存在的情况下，在100℃孵育10-15分钟后，它仍保持其相变逆转特性，并且不被DNase破坏。然而，受体转化逆转因子被核糖核酸酶处理而失活。这些结果表明糖皮质激素受体的激活是一个可逆的过程，并提示RNA参与其中。
The combination of partially purified, activated (nuclear binding) mammary glucocorticoid receptor with a macromolecule present in cytosol converts the receptor to a form with properties of native receptor. After salt activation of native receptor and subsequent purification by DEAE-cellulose chromatography, the activated receptor sediments in sucrose density gradients at 4.0S and binds (80–90%) ATP-agarose. Combination of this partially purified, activated receptor with a factor present in crude cytosol allows the receptor to sediment in the position of native receptor (8.4S). Furthermore, this receptor form, like native receptor, does not interact significantly with ATPagarose. The cytosolic factor responsible for this receptor conversion is macromolecular (MW > 5000) and does not interact with ATP-agarose. It retains its transformation reversal properties after incubation at 100°C for 10–15 minutes in the presence and absence of 8M urea/1% SDS/1mM DTT and is not destroyed by treatment with DNase. However, the receptor transformation reversal factor is inactivated by treatment with RNase. These results demonstrate that glucocorticoid receptor activation is a reversible process and suggest that RNA is involved.