Effects of calcium ions on titanium surfaces for bone regeneration
Effects of calcium ions on titanium surfaces for bone regeneration
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DOI：
10.1016/j.colsurfb.2015.04.006
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发表时间：
2015-06-01
影响因子：
5.8
通讯作者：
Tejero, Ricardo
中科院分区：
文献类型：
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作者：
Anitua, Eduardo;Pinas, Laura;Tejero, Ricardo
种植体表面的化学和形貌对于承重牙科和骨科种植体的成功组织整合至关重要。在这里，我们评估在体外和体内钛种植体表面改性钙离子（Ca 2+表面）。钙离子对氧化钛界面产生持久的化学和纳米形貌改性。飞行时间二次离子质谱检查的最外层表面组成，表明钙离子在Ca 2+表面有效地防止外来烃钝化的氧化物层。在水溶液中，Ca 2+表面在第一分钟内释放，占总测量Ca 2+的2/3，其余的在随后的85天内释放。此外，钙离子表面显着增加人胎儿成骨细胞样细胞的粘附，增殖和分化，骨桥蛋白的自分泌合成所测量。与临床应用相关的是，绵羊胫骨愈合12周后，微电脑断层扫描和组织形态学分析显示，在植入物周围1 mm区域，Ca 2+表面形成了显著更多的骨接触和更高的骨密度。因此，在临床实践中，用钙离子改性的钛种植体代表了改善骨内整合的有价值的工具。(C)2015 Elsevier B. V.版权所有。
The chemistry and topography of implant surfaces are of paramount importance for the successful tissue integration of load-bearing dental and orthopedic implants. Here we evaluate in vitro and in vivo titanium implant surfaces modified with calcium ions (Ca2+ surfaces). Calcium ions produce a durable chemical and nano-topographical modification of the titanium oxide interface. Time of flight secondary ion mass spectrometry examination of the outermost surface composition, shows that calcium ions in Ca2+ surfaces effectively prevent adventitious hydrocarbon passivation of the oxide layer. In aqueous solutions Ca2+ surfaces release within the first minute, 2/3 of the total measured Ca2+, the rest is released over the following 85 days. Additionally, Ca2+ surfaces significantly increase human fetal osteoblasts-like cell adhesion, proliferation and differentiation, as measured by the autocrine synthesis of osteopontin. Relevant for clinical application, after 12 weeks of healing in sheep tibia, microcomputer tomography and histomorphometric analysis show that Ca2+ surfaces develop significantly more bone contacts and higher bone density in the 1 mm region around the implant. Consequently, titanium implants modified with calcium ions represent a valuable tool to improve endosseous integration in the clinical practice. (C) 2015 Elsevier B.V. All rights reserved.