GENETIC VARIATION IN WALNUTS (JUGLANS REGIA AND J. SIGILLATA; JUGLANDACEAE): SPECIES DISTINCTIONS, HUMAN IMPACTS, AND THE CONSERVATION OF AGROBIODIVERSITY IN YUNNAN, CHINA
GENETIC VARIATION IN WALNUTS (JUGLANS REGIA AND J. SIGILLATA; JUGLANDACEAE): SPECIES DISTINCTIONS, HUMAN IMPACTS, AND THE CONSERVATION OF AGROBIODIVERSITY IN YUNNAN, CHINA
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DOI：
10.3732/ajb.0900114
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发表时间：
2010-04-01
影响因子：
3
通讯作者：
Hai Xian
中科院分区：
文献类型：
--
作者：
Gunn, Bee F.;Aradhya, Mallikarjuna;Hai Xian
核桃是许多国家的主要农作物，大多数种植在大规模种植园，品种很少。地方品种提供了重要的遗传库，因此，了解影响作物资源遗传变异地理分布的因素是农业生物多样性保护的一个基本目标。在这里，我们研究了人类住区和亲属关系对两个核桃物种的遗传变异和种群结构的作用：核桃，一个广泛种植的坚果引进种，和J. sigillata，一个本地种在云南种植。本研究利用14个分子标记对同域分布的核桃（J.regia）和核桃（J.sigillata）种群进行了分析，并评价了藏族村落和亲属群体（亲属户）对核桃（J.regia）和核桃（J.sigillata）基因型变异和种群结构的影响。我们的研究结果基于220个核桃树从6个藏族村庄显示，虽然核桃和核桃的形态不同，这两个物种是不可区分的微卫星数据。尽管缺乏种间差异，但AMOVAs在村庄（5.41%，P = 0.0068）和村庄内亲属组（3.34%，P = 0.0068）之间的分配显示出显着的遗传变异。这些结果表明，村落环境和家庭关系是影响西藏核桃遗传变异地理结构的因素。
Walnuts are a major crop of many countries and mostly cultivated in large-scale plantations with few cultivars. Landraces provide important genetic reservoirs; thus, understanding factors influencing the geographic distribution of genetic variation in crop resources is a fundamental goal of agrobiodiversity conservation. Here, we investigated the role of human settlements and kinship on genetic variation and population structure of two walnut species: Juglans regia, an introduced species widely cultivated for its nuts, and J. sigillata, a native species cultivated locally in Yunnan. The objectives of this study were to characterize sympatric populations of J. regia and J. sigillata using 14 molecular markers and evaluate the role of Tibetan villages and kin groups (related households) on genotypic variation and population structure of J. regia and J. sigillata. Our results based on 220 walnut trees from six Tibetan villages show that although J. regia and J. sigillata are morphologically distinct, the two species are indistinguishable based on microsatellite data. Despite the lack of interspecific differences, AMOVAs partitioned among villages (5.41%, P = 0.0068) and kin groups within villages (3.34%, P = 0.0068) showed significant genetic variation. These findings suggest that village environments and familial relationships are factors contributing to the geographic structure of genetic variation in Tibetan walnuts.