ANALYSIS OF MOLECULAR MARKERS AS IL-12, IL-22 AND IFN-γ IN CORRELATION WITH A CLINICAL COURSE IN PATIENTS WITH PSORIASIS
ANALYSIS OF MOLECULAR MARKERS AS IL-12, IL-22 AND IFN-γ IN CORRELATION WITH A CLINICAL COURSE IN PATIENTS WITH PSORIASIS
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DOI：
10.13075/ijomeh.1896.01513
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发表时间：
2020-01-01
影响因子：
2
通讯作者：
Wozniacka, Anna
中科院分区：
文献类型：
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作者：
Kutwin, Magdalena;Migdalska-Sek, Monika;Wozniacka, Anna
目的：银屑病是一种慢性、复发性、免疫调节的全身性疾病，也是皮肤病的常见病因，常成为科学研究的对象。位于手部的皮肤变化会导致专业活动，特别是手工活动的执行方面的困难和限制。在发病机制中，主要由免疫因素发挥作用-免疫系统的组成部分功能不正常，其中包括T淋巴细胞和细胞因子，如白细胞介素12(IL-12)、白细胞介素22(IL-22)和干扰素-伽马(干扰素-伽马)。材料和方法：获取组织和血液，用于RNA提取。然后对RNA进行逆转录反应。用实时定量聚合酶链式反应检测IL-12B、IL-22和干扰素-γ基因的相对表达水平，并以相对定量(RQ)值的形式表示。此外，通过银屑病面积和严重程度指数(PASI)、体表面积(BSA)和皮肤病生活质量指数(DLQI)评分，对所选基因的表达水平与疾病的临床病程进行相关性分析。结果：统计学分析证实银屑病患者血清IL-12B、IL-22和干扰素-γ的RQ值明显高于对照组。BSA与PASI、IL-12B基因RQ呈正相关。结论：IL-12B、IL-22和干扰素-γ基因在银屑病皮肤中的高表达证实了某些细胞因子在银屑病的发生和维持中起重要作用。
Objectives: As a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities, especially manual ones. The main role in pathogenesis is played by immunological factors - improper functioning of the components of the immune system, among others, T lymphocytes and cytokines like interleukin-12 (IL-12), interleukin-22 (IL-22) and interferon gamma (IFN-gamma). Material and Methods: The obtained tissue and blood were destined for RNA isolation. The RNA was then subjected to a reverse transcription reaction. The relative gene expression level was evaluated by the real-time polymerase chain reaction for IL-12B, IL-22 and IFN-gamma genes, and presented as the relative quantification (RQ) value, relative to the reference gene GAPDH. In addition, a correlation analysis of the expression level of selected genes with the clinical course of the disease, as assessed by the Psoriasis Area and Severity Index (PASI), the Body Surface Area (BSA) and the Dermatology Life Quality Index (DLQI) scores was performed. Results: Statistical analysis confirmed a significant increase in RQ values for IL-12B, IL-22 and IFN-gamma in the group of psoriatic patients vs. the control group. A positive correlation was also found between BSA and PASI and RQ for the IL-12B gene. Conclusions: Increased expression levels of IL-12B, IL-22 and IFN-gamma genes in psoriatic skin confirm that selected cytokines play an important role in the initiation and sustenance of psoriasis.