BR2 cell penetrating peptide improved the transfection efficiency of modified polyethyleneimine
BR2 cell penetrating peptide improved the transfection efficiency of modified polyethyleneimine
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DOI：
10.1016/j.jddst.2019.101154
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发表时间：
2019-10-01
影响因子：
5
通讯作者：
Malaekeh-Nikouei, Bizhan
中科院分区：
文献类型：
--
作者：
Akbarzadeh, Mahdi;Oskuee, Reza Kazemi;Malaekeh-Nikouei, Bizhan
聚乙烯亚胺（PEI）是基因传递中应用最广泛的聚合物之一。细胞穿透肽（CPPs）是一组可以将物质从细胞膜转移到细胞质和细胞核的肽。本研究的目的是用三种不同的CPPs作为阳离子聚合物修饰PEI，以研究和比较它们的基因传递效率。从质粒缩聚、缓冲能力和伯胺数量等方面对cpp改性聚合物进行了评价。在LNCAP细胞中检测制备的纳米颗粒的大小、zeta电位、转染效率和细胞毒性。得到的纳米颗粒的粒径在183到313纳米之间。测量了载体的zeta电位，表明纳米粒子的电荷在21 ~ 25 mV之间。载体可浓缩质粒，载体质粒比为4。转染LNCAP细胞系的结果表明，含有BR2肽的载体转染活性最好。在细胞毒性试验中，我们观察到载体质粒比为4时含有CyLoP1的载体细胞毒性最高。综上所述，由于聚合物的物理化学变化，CPPs与PEI的偶联导致转染活性提高。
Polyethyleneimine (PEI) is one of the most widely used polymers in gene delivery. Cell penetrating peptides (CPPs) are a group of peptides that can transfer materials from the membrane to the cytoplasm and nucleus. The aim of this study was to modify PEI with three different CPPs as a cationic polymer in order to investigate and compare their efficiency in gene delivery. The CPP-modified polymers were evaluated in terms of plasmid condensation, buffering capacity and number of primary amines. The prepared nanoparticles were examined for size, zeta potential, transfection efficiency and cytotoxicity in LNCAP cells. The resulting nanoparticles had a particle size between 183 and 313 nm. Measuring the zeta potential of the vectors indicated that the charge of the nanoparticles is between 21 and 25 mV. Vectors can condense plasmid in carrier-plasmid ratio of 4. Transfection results on the LNCAP cell line showed that the vector containing the BR2 peptide had the best transfection activity. In the cytotoxicity test, it was observed that vector containing CyLoP1 in carrier-plasmid ratio of 4 had the highest cell cytotoxicity. In conclusion, conjugation of CPPs to PEI due to physicochemical changes of the polymer resulted in improved transfection activity.