Permanent genetic memory with >-byte capacity
Permanent genetic memory with >-byte capacity
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发表时间：
2014
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通讯作者：
Lei Yang;Alec A. K. Nielsen;J. Fernandez-Rodriguez;Conor J. McClune;M. Laub;T. Lu;Christopher A. Voigt-Christopher-A.
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作者：
Lei Yang;Alec A. K. Nielsen;J. Fernandez-Rodriguez;Conor J. McClune;M. Laub;T. Lu;Christopher A. Voigt-Christopher-A.
nAture Methods|优先数字出版|遗传记忆使得能够在活细胞的DNA中记录信息。存储器可以记录瞬态环境信号或单元状态，然后在稍后的时间调用该瞬态环境信号或单元状态。永久性存储器使用不可逆重组酶来实现，该不可逆重组酶反转dnA的单位的方向，对应于位的[0，]状态。为了扩大记忆容量，我们应用生物信息学鉴定了34种噬菌体整合酶（及其同源的attB和attP识别位点），由此我们构建了彼此完全正交的记忆开关以及Fime和hbiF细菌转化酶。利用这些开关，在大肠杆菌中构建了一个存储器阵列，可以记录0.375字节的信息。证明了重组酶可以分层并用于永久记录转录逻辑门的瞬时状态。
nAture methods | ADVANCE ONLINE PUBLICATION | Genetic memory enables the recording of information in the dnA of living cells. memory can record a transient environmental signal or cell state that is then recalled at a later time. Permanent memory is implemented using irreversible recombinases that invert the orientation of a unit of dnA, corresponding to the [0, ] state of a bit. to expand the memory capacity, we have applied bioinformatics to identify 34 phage integrases (and their cognate attB and attP recognition sites), from which we build memory switches that are perfectly orthogonal to each other and the Fime and hbiF bacterial invertases. using these switches, a memory array is constructed in Escherichia coli that can record .375 bytes of information. it is demonstrated that the recombinases can be layered and used to permanently record the transient state of a transcriptional logic gate.