Plasmid-mediated mcr-1 gene in Acinetobacter baumannii and Pseudomonas aeruginosa: first report from Pakistan
Plasmid-mediated mcr-1 gene in Acinetobacter baumannii and Pseudomonas aeruginosa: first report from Pakistan
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DOI：
10.1590/0037-8682-0237-2019
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发表时间：
2019-01-01
影响因子：
2
通讯作者：
Rehman, Tayyab Ur
中科院分区：
文献类型：
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作者：
Hameed, Fareeha;Khan, Muhammad Asif;Rehman, Tayyab Ur
简介：粘菌素用于治疗鲍曼不动杆菌和铜绿假单胞菌引起的感染的增加导致了粘菌素耐药性。本研究的目的是检测质粒介导的mcr-1基因在耐粘菌素A中的表达。鲍曼不动杆菌和铜绿假单胞菌分离株。方法：对146株临床分离的A.鲍曼不动杆菌（n = 62）和铜绿假单胞菌（n = 84）从巴基斯坦白沙瓦的四家最大的三级护理医院收集。使用Kirby-Baur纸片扩散法对所有细菌分离株进行多药耐药表型筛选。使用稀释法进行所有分离株中粘菌素的最小抑菌浓度（MIC）的表型分析。采用聚合酶链反应检测mcr-1基因，桑格测序法测定扩增产物的核苷酸序列。结果：A.鲍曼不动杆菌和83.3%的铜绿假单胞菌对多种抗生素耐药。粘菌素耐药率为9.6%（6/62）。铜绿假单胞菌11.9%（10/84）。在16株粘菌素耐药菌株中，有1株检出mcr-1基因。鲍曼不动杆菌（占总分离株的1.61%;粘菌素耐药分离株的16.6%）和1株铜绿假单胞菌菌株（占总分离株的1.19%;粘菌素耐药分离株的10%）。与GenBank中的mcr-1基因序列同源性为98-99%。结论：我们的研究首次报道了质粒介导的mcr-1编码的粘菌素耐药性在多重耐药的A.鲍曼不动杆菌和铜绿假单胞菌。建议进一步进行大规模研究，以调查这些高致病性细菌中这种耐药模式的流行情况。
Introduction: The increased use of colistin against infections caused by Acinetobacter baumannii and Pseudomonas aeruginosa has resulted in colistin resistance. The purpose of this study was to detect plasmid-mediated mcr-1 gene in colistin-resistant A. baumannii and P. aeruginosa isolates. Methods: A total of 146 clinical isolates of A. baumannii (n = 62) and P. aeruginosa (n = 84) were collected from the four largest tertiary care hospitals in Peshawar, Pakistan. All bacterial isolates were phenotypically screened for multidrug resistance using the Kirby-Baur disc diffusion method. The minimum inhibitory concentration (MIC) of colistin in all isolates was phenotypically performed using dilution methods. mcr-1 gene was detected through polymerase chain reaction and the nucleotide sequence of amplicon was determined using Sanger sequencing. Results: Approximately 96.7% A. baumannii and 83.3% P. aeruginosa isolates were resistant to multiple antibiotics. Colistin resistance was found in 9.6% (6/62) of A. baumannii and 11.9% (10/84) of P. aeruginosa isolates. Among 16 colistin resistant isolates, the mcr-1 gene was detected in one A. baumannii (1.61% of total isolates; 16.6% of colistin resistant isolates) and one P. aeruginosa strain (1.19% of total isolates; 10% of colistin resistant isolates). Nucleotide BLAST showed 98-99% sequence similarity to sequences of the mcr-1 gene in GenBank. Conclusions: Our study reports, for the first time, the emergence of plasmid-mediated mcr-1-encoded colistin resistance in multidtug resistant strains of A. baumannii and P. aeruginosa. Further large scales studies are recommended to investigate the prevalence of this mode of resistance in these highly pathogenic bacteria.