Characterization of the human type 2 neuropeptide Y receptor gene (NPY2R) and localization to the chromosome 4q region containing the type 1 neuropeptide Y receptor gene
Characterization of the human type 2 neuropeptide Y receptor gene (NPY2R) and localization to the chromosome 4q region containing the type 1 neuropeptide Y receptor gene
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DOI：
10.1006/geno.1996.0642
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发表时间：
1996-12-15
期刊：
影响因子：
4.4
通讯作者：
Thompson, DA
中科院分区：
文献类型：
--
作者：
Ammar, DA;Eadie, DM;Thompson, DA
神经肽Y（neuropeptide Y，NPY）通过存在于脑和交感神经元中的一个G蛋白偶联受体家族传递信号。为了进一步了解参与NPY受体表达调控的遗传元件，我们克隆并鉴定了编码2型NPY受体（Y2 receptor，HGMW批准的符号NPY 2 R）的人类基因。(2)转录本跨越9 kb的基因组序列，并在两个外显子上编码。与1型NPY受体（Y1受体）基因一样，Y2受体的5 ′-非翻译区被间插序列（类似于4.5kb）中断。然而，Y2受体基因不含与Y1受体编码区中存在的内含子类似的内含子。预测的转录物大小（类似于4.5 kb）与通过北方分析观察到的大小一致。从开放阅读框推导的381个氨基酸的序列与cDNA编码的序列相同。Y2受体基因定位于人类染色体4 q31，与Y1受体基因座相同的区域，这表明这些亚型可能是由于基因重复而产生的，尽管它们的结构存在差异。(C)出版社：Academic Press，Inc.
Neuropeptide Y (NPY) signals through a family of G-protein coupled receptors present in the brain and sympathetic neurons, To further our understanding of the genetic elements involved in the regulation of NPY receptor expression, we have cloned and characterized the human gene encoding the type 2 NPY receptor (Y2 receptor, HGMW-approved symbol NPY2R).(2) The transcript spans 9 kb of genomic sequence and is encoded on two exons. As in the type 1 NPY receptor (YI receptor) gene, the 5'-untranslated region of the Y2 receptor is interrupted by an intervening sequence (similar to 4.5 kb), However, the Y2 receptor gene does not contain an intron analogous to that present in the coding region of the Y1 receptor. The predicted transcript size (similar to 4.5 kb) is consistent with the size observed by Northern analysis. The 381-amino-acid sequence deduced from the open reading frame is identical to that encoded by the cDNA. The Y2 receptor gene maps to human chromosome 4q31, the same region containing the Y1 receptor locus, suggesting that these subtypes may have arisen by gene duplication despite their structural differences. (C) 1996 Academic Press, Inc.