Genotype identification and prevalence of Giardia duodenalis in pet dogs of Guangzhou, Southern China
Genotype identification and prevalence of Giardia duodenalis in pet dogs of Guangzhou, Southern China
复制标题
广州地区宠物犬十二指肠贾第鞭毛虫基因型鉴定及流行情况
DOI：
10.1016/j.vetpar.2012.04.004
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发表时间：
2012-09-10
影响因子：
2.6
通讯作者：
Li, Guoqing
中科院分区：
文献类型：
--
作者：
Li, Jie;Zhang, Ping;Li, Guoqing
贾第鞭毛虫是一种鞭毛寄生虫，被认为是世界范围内人类和动物原虫腹泻的最常见原因之一。本文首次对G.中国广州宠物狗体内的寄生虫。粪便样本（209个标本）从年轻人（3岁）。粪便标本镜检阳性率为8.61%（18/209），PCR阳性率为11.00%（23/209）。幼龄犬的患病率（25.58%）高于成年犬（7.37%）和老年犬（7.04%），差异有统计学意义（P < 0.05）。公犬患病率11.30%（13/115）高于母犬10.87%（10/92），郊区犬患病率12.15%高于市区犬9.80%，但差异无统计学意义（P > 0.05）。对23个PCR阳性样品进行序列分析，发现D组合（18/23）和人畜共患病A组合（5/23）。本研究报告了高感染率的G。在宠物狗中，特别是在年轻的狗。基因型特征表明，存在人畜共患病组合A，这一事实构成了G的潜在风险。从宠物狗到人类的尿道炎传播。建议宠物主人采取适当的卫生措施，预防和控制该地区贾第鞭毛虫病。(C)2012爱思唯尔有限公司版权所有。
Giardia duodenalis is a flagellated parasite and is considered one of the most common causes of protozoal diarrhea in both humans and animals worldwide. This paper represents the first study of the prevalence of G. duodenalis in pet dogs in Guangzhou, China. Faecal samples (209 specimens) were obtained from young (3 years old). 8.61% (18/209) faecal samples were recorded positive using microscopy examination, and 11.00% (23/209) using PCR. The prevalence was significantly higher in diarrheic dogs (26.31%) compared with non-diarrheic dogs (5.10%), while it was higher in young (25.58%) than both adult (7.37%) and elder (7.04%) dogs and the difference was statistically significant (P < 0.05). The prevalence in male dogs 11.30% (13/115) was higher than females 10.87% (10/92), and in suburban dogs (12.15%) higher than urban 9.80%, but the difference was not statistically significant (P > 0.05). Sequence analysis of the 23 PCR-positive samples revealed the presence of Assemblage D (18/23), and zoonotic Assemblage A (5/23). The present investigation reported a high infection rate of G. duodenalis in pet dogs, especially in young dogs. Genotypic characterization demonstrated that the zoonotic Assemblage A was found, a fact that poses a potential risk of G. duodenalis transmission from pet dogs to humans. It is suggested that pet owners should take appropriate hygiene measures to prevent and control giardiasis in this region. (C) 2012 Elsevier B.V. All rights reserved.